Momin V M
Z Mikrosk Anat Forsch. 1975;89(4):744-57.
The leg muscle of Calotes versicolor consists mainly of white fibers. They can be classified into small, intermediate and broad fibers according to their diameter and staining reaction. The histochemical study of some of the glycolytic enzymes such as aldolase, phosphorylase, lactic dehydrogenase (LDH) and alpha-glycerophosphate dehydrogenase (alpha-GPDH) is described in the muscle of normal, pancreactomized and insulin injected animals. Highest activity of all enzymes was observed in the small fibers, though they occur in negligiable amount (3%). The intermediate and broad fibers exhibited medial and lowest activities respectively. 24 h after the extirpation of the pancreas a considerable inhibition is noted in the activities of phosphorylase, LDH and alpha-GPDH. Induced inanition for 21 days ensued insignificant but gradual decrease and increase in the alpha-GPDH and phosphorylase activities respectively in both unoperated and operated animals. The aldolase shows its mere presence in normal and trace activity in pancreatectomized animals. The injection of insulin (40 i.u./kg) at the interval of 30 min, 60 min, 4 h, 12 h and 24 h is given. The activities of all enzymes is stimulated at 30 min and depleted 4 h after the injection. The level of activity was remaintained at 12 h to 24 h stage. The possible physiological role and significance of enzymes in the synthesis and breakdown of glycogen is discussed.
变色树蜥的腿部肌肉主要由白色纤维组成。根据直径和染色反应，它们可分为细纤维、中间纤维和粗纤维。本文描述了在正常动物、胰腺切除动物和注射胰岛素动物的肌肉中，对一些糖酵解酶如醛缩酶、磷酸化酶、乳酸脱氢酶（LDH）和α-甘油磷酸脱氢酶（α-GPDH）的组织化学研究。所有酶在细纤维中的活性最高，尽管细纤维数量极少（占3%）。中间纤维和粗纤维的活性分别为中等和最低。胰腺切除24小时后，磷酸化酶、LDH和α-GPDH的活性受到显著抑制。在未手术和手术动物中，诱导禁食21天分别导致α-GPDH和磷酸化酶活性出现不显著但逐渐的降低和升高。醛缩酶在正常动物中仅微量存在，在胰腺切除动物中活性极低。每隔30分钟、60分钟、4小时、12小时和24小时注射胰岛素（40国际单位/千克）。注射后30分钟时所有酶的活性均受到刺激，4小时后活性降低。在12小时至24小时阶段活性水平保持不变。本文还讨论了这些酶在糖原合成和分解中的可能生理作用及意义。
