血清学阳性公牛精液中无牛白血病病毒。
Absence of bovine leukosis virus in semen of seropositive bulls.
作者信息
Choi K Yeon, Monke Don, Stott Jeffrey L
机构信息
Investigen, Inc, Alameda, CA, USA.
出版信息
J Vet Diagn Invest. 2002 Sep;14(5):403-6. doi: 10.1177/104063870201400507.
A polymerase chain reaction (PCR)-based detection system was established to identify the presence of bovine leukosis virus (BLV) DNA in bovine semen. Seventy-nine bulls were included in the study. Serum, peripheral blood leukocytes, and semen were collected from each of the 79 bulls. The BLV-specific antibody was detected in serum by agar gel immunodiffusion and viral DNA in blood and semen by PCR. Serologically, 29 of the 79 bulls were BLV positive. Twenty-seven of the 29 seropositive bulls and 1 of the seronegative bulls had BLV DNA in peripheral blood leukocytes. All 79 bulls tested PCR negative for the presence of BLV in semen. This data is strong evidence that properly collected semen from BLV seropositive bulls will not contribute to dissemination of this viral infection.
建立了一种基于聚合酶链反应（PCR）的检测系统，以鉴定牛精液中牛白血病病毒（BLV）DNA的存在。该研究纳入了79头公牛。从这79头公牛中的每一头采集血清、外周血白细胞和精液。通过琼脂凝胶免疫扩散检测血清中的BLV特异性抗体，通过PCR检测血液和精液中的病毒DNA。血清学检测显示，79头公牛中有29头BLV呈阳性。29头血清阳性公牛中的27头以及1头血清阴性公牛的外周血白细胞中存在BLV DNA。所有79头公牛的精液经PCR检测均未发现BLV。这些数据有力地证明，从BLV血清阳性公牛正确采集的精液不会导致这种病毒感染的传播。
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