[使用诊断性DNA探针检测感染牛白血病病毒的奶牛中的前病毒]
[Use of diagnostic DNA probes for detection of the provirus of bovine leukemia virus in infected cows].
作者信息
Mal'tseva N A, Kaledin A S, Oliĭnik E I, Zhivotov A A, Baranov V I, Karelin V P
出版信息
Vopr Virusol. 2002 Nov-Dec;47(6):38-41.
A method of detection of BLV proviral DNA in the peripheral blood leukocytes of cattle is reported. Leukocytes were used without preliminary cultivation. Cell DNA was dot-hybridized with P-labeled plasmid containing a fragment of BLV proviral DNA. The sera samples taken from the cattle under study were also tested using immunodiffusion assay (ID). The results of comparison showed that dot-hybridization assay is a more sensitive diagnostic test than ID, because it detects BLV infection in apparently normal cattle, which was seronegative in ID.
本文报道了一种检测牛外周血白细胞中BLV前病毒DNA的方法。白细胞未经预先培养直接使用。细胞DNA与含BLV前病毒DNA片段的P标记质粒进行斑点杂交。对所研究牛采集的血清样本也采用免疫扩散试验（ID）进行检测。比较结果表明，斑点杂交试验是比免疫扩散试验更灵敏的诊断方法，因为它能检测出在免疫扩散试验中血清学呈阴性但看似正常的牛的BLV感染。
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