Santra Madhumita, Das Swapan Kumar, Talukder Geeta, Sharma Archana
Department of Pathology, Vivekananda Institute of Medical Sciences, Calcutta, India.
Biol Trace Elem Res. 2002 Aug;88(2):139-44. doi: 10.1385/BTER:88:2:139.
In the present study, we report the results of the capability of zinc chloride for the induction of micronuclei in cultured human leukocytes using cytokinesis-block micronucleus assay. Two concentrations of zinc chloride (1.5 x 10(-4) M and 3.0 x 10(-4) M) were used to evaluate the potential of this zinc salt to induce micronucleus formation. This effect was compared with positive (mitomycin C treated) and negative controls (no salt added). Our results show a significant (p < or = 0.001) increase of micronucleated cytokinesis-blocked cells (MNCBs) in zinc-chloride-treated cells compared to the negative control. Induction of MNCBs was not in a dose-dependent manner for zinc chloride concentrations tested. This report is the first to describe the efficiency of cytokinesis-block micronucleus assay to evaluate the genotoxic effects of zinc salt.
在本研究中，我们报告了使用胞质分裂阻断微核试验检测氯化锌诱导培养的人白细胞中微核形成能力的结果。使用两种浓度的氯化锌（1.5×10⁻⁴ M和3.0×10⁻⁴ M）来评估这种锌盐诱导微核形成的潜力。将这种效应与阳性对照（丝裂霉素C处理）和阴性对照（未添加盐）进行比较。我们的结果显示，与阴性对照相比，氯化锌处理的细胞中微核化的胞质分裂阻断细胞（MNCBs）显著增加（p≤0.001）。在所测试的氯化锌浓度下，MNCBs的诱导并非呈剂量依赖性。本报告首次描述了胞质分裂阻断微核试验评估锌盐遗传毒性效应的效率。
