Santra M, Talukder G, Sharma A
Department of Pathology, Vivekananda Institute of Medical Sciences, Calcutta, India.
Biol Trace Elem Res. 2000 Winter;78(1-3):113-9. doi: 10.1385/BTER:78:1-3:113.
The degree of chromosome damage induced by three compounds of zinc (zinc chloride, zinc sulfate, zinc acetate) was compared in human leucocytes in vitro. Three concentrations of each salt, 3.0 x 10(-5)M, 3.0 x 10(-4)M, and 1.5 x 10(-3)M, were added to leukocyte cultures. The cells were harvested after 48 and 72 h and chromosome spreads were prepared following a colchicine-hypotonic-fixation-air drying-Giemsa staining schedule. The end point screened was chromosome aberrations. All three salts were lethal at the highest concentration. The degree of chromosome damage was directly proportional to the concentrations used for zinc sulfate and zinc acetate but not for zinc chloride.
在体外对人白细胞中三种锌化合物（氯化锌、硫酸锌、醋酸锌）诱导的染色体损伤程度进行了比较。每种盐的三个浓度，即3.0×10⁻⁵M、3.0×10⁻⁴M和1.5×10⁻³M，被添加到白细胞培养物中。在48小时和72小时后收获细胞，并按照秋水仙碱-低渗-固定-空气干燥-吉姆萨染色程序制备染色体涂片。筛选的终点是染色体畸变。所有三种盐在最高浓度时都是致死的。硫酸锌和醋酸锌的染色体损伤程度与所用浓度成正比，但氯化锌并非如此。
