Schlappi M., Hohn B.
Friedrich Miescher-Institut, CH-4002 Basel, Switzerland.
Plant Cell. 1992 Jan;4(1):7-16. doi: 10.1105/tpc.4.1.7.
Agrobacterium-mediated transfer of viral sequences to plant cells (agroinfection) was applied to study the susceptibility of immature maize embryos to the pathogen. The shoot apical meristem of immature embryos 10 to 20 days after pollination from four different maize genotypes was investigated for competence for agroinfection. There was a direct correlation between different morphological stages of the unwounded immature embryos and their competence for agroinfection. Agroinfection frequency was highest in the embryogenic line A188. All developmental stages tested showed Agrobacterium virulence gene-inducing activity, whereas bacteriocidal substances were produced at stages of the immature embryos competent for agroinfection. The results suggested that Agrobacterium may require differentiated tissue in the maize shoot apical meristem before wounding for successful T-DNA transfer. This requirement for the young maize embryo has implications for the possible use of Agrobacterium for maize transformation.
采用农杆菌介导的病毒序列向植物细胞转移(农杆菌介导的感染)来研究未成熟玉米胚对病原体的易感性。对四种不同玉米基因型授粉后10至20天的未成熟胚的茎尖分生组织进行农杆菌介导的感染能力研究。未受伤的未成熟胚的不同形态阶段与其农杆菌介导的感染能力之间存在直接相关性。在胚性系A188中农杆菌介导的感染频率最高。所有测试的发育阶段均显示出农杆菌毒力基因诱导活性,而在能够进行农杆菌介导的感染的未成熟胚阶段会产生杀菌物质。结果表明,农杆菌可能需要在未成熟玉米茎尖分生组织中存在分化组织,然后再进行创伤,才能成功进行T-DNA转移。对年轻玉米胚的这种要求对农杆菌用于玉米转化的可能性具有启示意义。