T-DNA 转移至玉米植株。
T-DNA transfer to maize plants.
作者信息
Shen W H, Escudero J, Hohn B
机构信息
IBMP/CNRS, Strasbourg, France.
出版信息
Mol Biotechnol. 1999 Dec 1;13(2):165-70. doi: 10.1385/MB:13:2:165.
PMID:10934530
Abstract
Agrobacterium-mediated transformation is the method of choice to engineer desirable genes into plants. Here we describe a protocol for demonstrating T-DNA transfer from Agrobacterium into the economically important graminaceous plant maize. Expression of the T-DNA-located GUS gene was observed with high efficiency on shoots of young maize seedlings after cocultivation with Agrobacterium.
摘要
农杆菌介导的转化是将所需基因导入植物的首选方法。在此，我们描述了一种用于证明T-DNA从农杆菌转移到具有重要经济价值的禾本科植物玉米中的实验方案。在用农杆菌共培养后，在玉米幼苗的芽上高效观察到了位于T-DNA上的GUS基因的表达。
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