Seeley K. A., Byrne D. H., Colbert J. T.
Department of Botany, Iowa State University, Ames, Iowa 50011.
Plant Cell. 1992 Jan;4(1):29-38. doi: 10.1105/tpc.4.1.29.
Phytochrome A (phyA) mRNA abundance decreased rapidly in total RNA samples isolated from 4-day-old etiolated oat seedlings following a red light pulse. Putative in vivo phyA mRNA degradation products were detectable both before and after red light treatment. Cordycepin-treated coleoptiles were unable to accumulate the chlorophyll a/b-binding protein mRNA in response to red light, indicating that cordycepin effectively inhibited mRNA synthesis. In cordycepin-treated coleoptiles, phyA mRNA rapidly decreased in abundance, consistent with the hypothesis that phyA mRNA is inherently unstable, rather than being destabilized after red light treatment of etiolated oat seedlings.
在红光脉冲处理后,从4日龄黄化燕麦幼苗中分离得到的总RNA样本中,光敏色素A(phyA)的mRNA丰度迅速下降。在红光处理前后均能检测到假定的体内phyA mRNA降解产物。经虫草素处理的胚芽鞘无法响应红光积累叶绿素a/b结合蛋白mRNA,这表明虫草素有效地抑制了mRNA的合成。在经虫草素处理的胚芽鞘中,phyA mRNA的丰度迅速下降,这与phyA mRNA本身不稳定的假设一致,而不是在黄化燕麦幼苗经红光处理后才变得不稳定。