Higgs D C, Barnes L J, Colbert J T
Department of Botany, Iowa State University, Ames 50011, USA.
Plant Mol Biol. 1995 Oct;29(2):367-77. doi: 10.1007/BF00043659.
Gene-preferential oligonucleotide probes were used to determined the relative abundance and half-lives of distinct oat phytochrome A (PHYA) mRNAs. Oat PHYA mRNAs are highly conserved in the 5'-untranslated region and the coding region, but the 3'-untranslated region has an overall lower sequence conservation and was the source of gene-preferential probes. PHYA3 mRNA was estimated to be ca. 61% of the oat PHYA mRNA pool present in poly(A)+ RNA from dark-grown seedlings. The half-lives for PHYA3 and PHYA4 mRNAs were both estimated to be ca. 30 min, and a similar short half-life was estimated for the average PHYA mRNA. Sequence comparisons of PHYA mRNAs from four grass species identified conserved sequences within the 5'- and 3'-untranslated regions that might be important for PHYA mRNA degradation.
基因特异性寡核苷酸探针被用于测定不同燕麦光敏色素A（PHYA）mRNA的相对丰度和半衰期。燕麦PHYA mRNA在5'-非翻译区和编码区高度保守，但3'-非翻译区的序列保守性总体较低，是基因特异性探针的来源。据估计，PHYA3 mRNA约占黑暗生长幼苗的聚腺苷酸加尾（poly(A)+）RNA中燕麦PHYA mRNA库的61%。PHYA3和PHYA4 mRNA的半衰期均估计约为30分钟，并且估计平均PHYA mRNA也有类似的短半衰期。对四种禾本科植物的PHYA mRNA进行序列比较，确定了5'-和3'-非翻译区内可能对PHYA mRNA降解很重要的保守序列。
