Gerbase-DeLima M, Meredith P, Walford R L
Fed Proc. 1975 Feb;34(2):159-61.
The mixed lymphocyte culture reaction represents the in vitro counterpart of the recognition phase of the graft-versus-host reaction, and of allograft rejection. The mixed lymphocyte culture reactivities of lymph node and spleen cells from all strains show a striking decline with advanced age. Furthermore, studies of "synergy" between subpopulations of T cells in the mixed lymphocyte culture reaction suggest that the cells of the recirculating lymphoid pool (T2 cells) in particular display a functional decline. Finally, spleen cells from old mice of appropriate strains inhibit or suppress the mixed lymphocyte culture reactivity of lymph node or spleen cells from young mice.
混合淋巴细胞培养反应代表了移植物抗宿主反应和同种异体移植排斥反应识别阶段的体外对应反应。所有品系的淋巴结和脾细胞的混合淋巴细胞培养反应性随着年龄的增长而显著下降。此外，对混合淋巴细胞培养反应中T细胞亚群之间“协同作用”的研究表明，特别是再循环淋巴细胞池（T2细胞）的细胞表现出功能衰退。最后，来自适当品系老年小鼠的脾细胞会抑制或压制来自幼年小鼠的淋巴结或脾细胞的混合淋巴细胞培养反应性。
