老年人外周淋巴细胞对植物血凝素增殖反应受损的细胞动力学分析。
Cytokinetic analysis of the impaired proliferative response of peripheral lymphocytes from aged humans to phytohemagglutinin.
作者信息
Tice R R, Schneider E L, Kram D, Thorne P
出版信息
J Exp Med. 1979 May 1;149(5):1029-41. doi: 10.1084/jem.149.5.1029.
Abstract
The effect of donor age on the rate of cell entry into the proliferating pool and subsequent cell cycle duration for peripheral lymphocytes stimulated by phytohemagglutinin (PHA) were examined by using the bromodeoxyuridine incorporation-differential staining technique. Distribution curves for the appearance of metaphase cells in successive generations as a function of culture time were obtained and analyzed both graphically and by a computer simulation model. Peripheral lymphocytes from aged individuals (approximately 75 yr) were stimulated by PHA at approximately one-half of the rate of peripheral lymphocytes from young individuals (approximately 21 yr). Subsequent cell-cycle durations were estimated to range from 10.0 to 25.0 h for aged individual lymphocyte cultures and 10.6-15.6 h for young individual lymphocyte cultures. The possible significance of these findings to aging in general is discussed.
摘要
采用溴脱氧尿苷掺入-差异染色技术，研究了供体年龄对植物血凝素（PHA）刺激的外周淋巴细胞进入增殖池的速率以及随后细胞周期持续时间的影响。获得了连续几代中期细胞出现的分布曲线，该曲线是培养时间的函数，并通过图形和计算机模拟模型进行了分析。老年个体（约75岁）的外周淋巴细胞受PHA刺激的速率约为年轻个体（约21岁）外周淋巴细胞的一半。老年个体淋巴细胞培养的后续细胞周期持续时间估计为10.0至25.0小时，年轻个体淋巴细胞培养的为10.6 - 15.6小时。讨论了这些发现对一般衰老可能具有的意义。
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