Malakian A H, Kaloustian S
Immunology. 1975 Jan;28(1):103-12.
A cytoplasmic component from group A streptococci produced complete suppression of human lymphocyte transformation induced by phytohaemagglutinin or the mixed lymphocyte reaction in vitro. It also suppressed antibody-forming cells in mice against sheep erythrocytes. The active substance was eluted as second and third fractions form Sephadex G-200 chromatography of the 100,000 g supernatant of sonically ruptured group A streptococci. The antimitogenic activity was not susceptible to trypsin, pronase, RNase or DNase digestion, but the activity was completely lost when it was sequentially digested, first with RNase and DNase and then with pronase. The active substance was not antigenic nor heat-labile at 56 degrees. It may be a protein component of a nucleoprotein.
A组链球菌的一种细胞质成分在体外可完全抑制由植物血凝素诱导的人淋巴细胞转化或混合淋巴细胞反应。它还能抑制小鼠体内针对绵羊红细胞的抗体形成细胞。活性物质从经超声破碎的A组链球菌100,000g上清液经Sephadex G - 200层析洗脱为第二和第三组分。抗有丝分裂活性对胰蛋白酶、链霉蛋白酶、核糖核酸酶或脱氧核糖核酸酶消化不敏感，但当它先用核糖核酸酶和脱氧核糖核酸酶消化，然后用链霉蛋白酶消化时，活性完全丧失。活性物质无抗原性，在56摄氏度时也不耐热。它可能是一种核蛋白的蛋白质成分。
