Nagura Taizo, Hachimura Satoshi, Hashiguchi Masaaki, Ueda Yoshihiro, Kanno Takashi, Kikuchi Hiroto, Sayama Kouji, Kaminogawa Shuichi
Department of Applied Biological Chemistry, The University of Tokyo, Bunkyo, Tokyo, Japan.
Br J Nutr. 2002 Oct;88(4):421-6. doi: 10.1079/BJN2002666.
The effects of the dietary oligosaccharide raffinose on immune responses, with special reference to its anti-allergic functions, were examined in vivo. First, feeding a diet supplemented with 50 g raffinose/kg to BALB/c mice significantly (P<0.05) increased interleukin (IL) 12 secretion from isolated Peyer's patch (PP) cells in vitro compared with feeding control diet. When isolated PP cells were used as antigen-presenting cells (APC) for CD4+ T-splenocytes isolated from ovalbumin (OVA)-specific T-cell receptor transgenic (Tg) mice in the presence of OVA as antigen, significantly (P<0.05) higher levels of interferon-gamma were observed in the cultures using APC from raffinose-fed mice than those cultures using APC from control mice. Second, the diet containing 50 g raffinose/kg or control diet was fed to OVA Tg mice, and subsequently, OVA was added to each diet to prime T cells in vivo. CD4+ T-cells from the mesenteric lymph nodes of the raffinose-fed mice secreted significantly (P<0.05) higher levels of IL-2 and significantly (P<0.05) lower levels of IL-4 following in vitro antigenic stimulation compared with those of the control mice. These present results suggest that feeding raffinose may suppress differentiation of naïve T-helper (Th) cells into Th2 cells in the mesenteric lymphoid nodes. Last, feeding raffinose suppressed rises of serum immunoglobulin E levels in the Tg mice treated with long-term ingestion of OVA. In conclusion, it is suggested that dietary raffinose suppresses serum immunoglobulin E response through suppression of Th2-type immune response against oral antigen in the lymphoid organs located in or near the intestine.
在体内研究了膳食低聚糖棉子糖对免疫反应的影响，特别关注其抗过敏功能。首先，与喂食对照饮食相比，给BALB/c小鼠喂食添加50 g棉子糖/kg的饮食，显著（P<0.05）增加了体外分离的派尔集合淋巴结（PP）细胞白细胞介素（IL）-12的分泌。当在存在卵清蛋白（OVA）作为抗原的情况下，将分离的PP细胞用作从OVA特异性T细胞受体转基因（Tg）小鼠分离的CD4+脾T细胞的抗原呈递细胞（APC）时，与使用来自对照小鼠的APC的培养物相比，在使用来自棉子糖喂养小鼠的APC的培养物中观察到显著（P<0.05）更高水平的干扰素-γ。其次，将含有50 g棉子糖/kg的饮食或对照饮食喂给OVA Tg小鼠，随后，将OVA添加到每种饮食中以在体内启动T细胞。与对照小鼠相比，体外抗原刺激后，来自棉子糖喂养小鼠肠系膜淋巴结的CD4+ T细胞分泌显著（P<0.05）更高水平的IL-2和显著（P<0.05）更低水平的IL-4。这些结果表明，喂食棉子糖可能会抑制肠系膜淋巴结中幼稚T辅助（Th）细胞向Th2细胞的分化。最后，喂食棉子糖抑制了长期摄入OVA处理的Tg小鼠血清免疫球蛋白E水平的升高。总之，提示膳食棉子糖通过抑制肠道内或附近淋巴器官中针对口服抗原的Th2型免疫反应来抑制血清免疫球蛋白E反应。
