通过属特异性聚合酶链反应-变性梯度凝胶电泳检测小鼠下肠道中的幽门螺杆菌定植情况。
Detection of Helicobacter colonization of the murine lower bowel by genus-specific PCR-denaturing gradient gel electrophoresis.
作者信息
Grehan Martin, Tamotia Gauri, Robertson Bronwyn, Mitchell Hazel
机构信息
School of Microbiology and Immunology, The University of New South Wales, Sydney, New South Wales, Australia 2052.
出版信息
Appl Environ Microbiol. 2002 Oct;68(10):5164-6. doi: 10.1128/AEM.68.10.5164-5166.2002.
Abstract
Helicobacter genus-specific PCR and denaturing gradient gel electrophoresis can detect and speciate the helicobacters that colonize the lower bowel of laboratory mice. The method's sensitivity is comparable to that of species-specific PCR and may detect unnamed Helicobacter species. This approach should prove useful for commercial and research murine facilities.
摘要
幽门螺杆菌属特异性聚合酶链反应（PCR）和变性梯度凝胶电泳可检测并鉴定在实验小鼠下消化道定植的幽门螺杆菌。该方法的灵敏度与种特异性PCR相当，且可能检测到未命名的幽门螺杆菌物种。这种方法对商业和研究性小鼠饲养设施应会有用。
相似文献
1
Detection of Helicobacter colonization of the murine lower bowel by genus-specific PCR-denaturing gradient gel electrophoresis.
Appl Environ Microbiol. 2002 Oct;68(10):5164-6. doi: 10.1128/AEM.68.10.5164-5166.2002.
2
Template DNA ratio can affect detection by genus-specific PCR-denaturing gradient gel electrophoresis of bacteria present at low abundance in mixed populations.
Helicobacter. 2005 Feb;10(1):80-2. doi: 10.1111/j.1523-5378.2005.00294.x.
3
High prevalence of Helicobacter Species detected in laboratory mouse strains by multiplex PCR-denaturing gradient gel electrophoresis and pyrosequencing.
J Clin Microbiol. 2004 Aug;42(8):3781-8. doi: 10.1128/JCM.42.8.3781-3788.2004.
4
Fecal PCR assay for diagnosis of Helicobacter infection in laboratory rodents.
J Clin Microbiol. 1997 Jun;35(6):1620-3. doi: 10.1128/jcm.35.6.1620-1623.1997.
5
Assessment of PCR-DGGE for the identification of diverse Helicobacter species, and application to faecal samples from zoo animals to determine Helicobacter prevalence.
J Med Microbiol. 2003 Sep;52(Pt 9):765-771. doi: 10.1099/jmm.0.05314-0.
6
Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species.
J Clin Microbiol. 2008 May;46(5):1867-9. doi: 10.1128/JCM.00139-08. Epub 2008 Mar 12.
7
Identification of murine helicobacters by PCR and restriction enzyme analyses.
J Clin Microbiol. 1996 Apr;34(4):942-6. doi: 10.1128/jcm.34.4.942-946.1996.
8
Helicobacter muricola sp. nov., a novel Helicobacter species isolated from the ceca and feces of Korean wild mouse (Mus musculus molossinus).
FEMS Microbiol Lett. 2002 Mar 19;209(1):45-51. doi: 10.1111/j.1574-6968.2002.tb11107.x.
9
A novel enterohepatic Helicobacter species 'Helicobacter mastomyrinus' isolated from the liver and intestine of rodents.
Helicobacter. 2005 Feb;10(1):59-70. doi: 10.1111/j.1523-5378.2005.00292.x.
10
Fluorogenic PCR-based quantitative detection of a murine pathogen, Helicobacter hepaticus.
J Clin Microbiol. 2001 Jul;39(7):2598-602. doi: 10.1128/JCM.39.7.2598-2602.2001.
引用本文的文献
1
DNA-based culture-independent analysis detects the presence of group a streptococcus in throat samples from healthy adults in Japan.
BMC Microbiol. 2016 Oct 11;16(1):237. doi: 10.1186/s12866-016-0858-5.
2
The microaerophilic microbiota of de-novo paediatric inflammatory bowel disease: the BISCUIT study.
PLoS One. 2013;8(3):e58825. doi: 10.1371/journal.pone.0058825. Epub 2013 Mar 12.
3
Enterohepatic helicobacter in ulcerative colitis: potential pathogenic entities?
PLoS One. 2011 Feb 23;6(2):e17184. doi: 10.1371/journal.pone.0017184.
4
Enterohepatic Helicobacter species are prevalent in mice from commercial and academic institutions in Asia, Europe, and North America.
J Clin Microbiol. 2007 Jul;45(7):2166-72. doi: 10.1128/JCM.00137-07. Epub 2007 May 16.
5
Nongastric Helicobacter species detected in the intestinal tract of children.
J Clin Microbiol. 2006 Jun;44(6):2276-9. doi: 10.1128/JCM.02017-05.
6
High prevalence of Helicobacter Species detected in laboratory mouse strains by multiplex PCR-denaturing gradient gel electrophoresis and pyrosequencing.
J Clin Microbiol. 2004 Aug;42(8):3781-8. doi: 10.1128/JCM.42.8.3781-3788.2004.
本文引用的文献
1
Monoassociation of SCID mice with Helicobacter muridarum, but not four other enterics, provokes IBD upon receipt of T cells.
Gastroenterology. 2002 May;122(5):1346-54. doi: 10.1053/gast.2002.32959.
2
Molecular diversity of Lactobacillus spp. and other lactic acid bacteria in the human intestine as determined by specific amplification of 16S ribosomal DNA.
Appl Environ Microbiol. 2002 Jan;68(1):114-23. doi: 10.1128/AEM.68.1.114-123.2002.
3
Helicobacter typhlonius sp. nov., a Novel Murine Urease-Negative Helicobacter Species.
J Clin Microbiol. 2001 Nov;39(11):3920-6. doi: 10.1128/JCM.39.11.3920-3926.2001.
4
Helicobacter ganmani sp. nov., a urease-negative anaerobe isolated from the intestines of laboratory mice.
Int J Syst Evol Microbiol. 2001 Sep;51(Pt 5):1881-1889. doi: 10.1099/00207713-51-5-1881.
5
Helicobacter hepaticus infection triggers inflammatory bowel disease in T cell receptor alphabeta mutant mice.
Comp Med. 2000 Dec;50(6):586-94.
6
Bifidobacterial diversity in human feces detected by genus-specific PCR and denaturing gradient gel electrophoresis.
Appl Environ Microbiol. 2001 Feb;67(2):504-13. doi: 10.1128/AEM.67.2.504-513.2001.
7
'Flexispira rappini' strains represent at least 10 Helicobacter taxa.
Int J Syst Evol Microbiol. 2000 Sep;50 Pt 5:1781-1787. doi: 10.1099/00207713-50-5-1781.
8
9
GenBank.
Nucleic Acids Res. 2000 Jan 1;28(1):15-8. doi: 10.1093/nar/28.1.15.
10
Enteric lesions in SCID mice infected with "Helicobacter typhlonicus," a novel urease-negative Helicobacter species.
Lab Anim Sci. 1999 Oct;49(5):496-505.
Zotero 插件