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本文引用的文献

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Prevalence of Helicobacter equorum in faecal samples from horses and humans.马和人粪便样本中马胃螺旋杆菌的患病率。
Vet Microbiol. 2007 Apr 15;121(3-4):378-83. doi: 10.1016/j.vetmic.2006.12.014. Epub 2006 Dec 22.
2
Development of a nested PCR method targeting a unique multicopy element, ISMap02, for detection of Mycobacterium avium subsp. paratuberculosis in fecal samples.开发一种针对独特多拷贝元件ISMap02的巢式PCR方法，用于检测粪便样本中的副结核分枝杆菌。
J Clin Microbiol. 2005 Sep;43(9):4744-50. doi: 10.1128/JCM.43.9.4744-4750.2005.
3
Natural and experimental Helicobacter infections.自然和实验性幽门螺杆菌感染
Comp Med. 2004 Apr;54(2):128-58.
4
Multiplex PCR assay for differentiation of Helicobacter felis, H. bizzozeronii, and H. salomonis.用于区分猫螺杆菌、比氏螺杆菌和所罗门螺杆菌的多重聚合酶链反应检测法。
J Clin Microbiol. 2004 Mar;42(3):1115-22. doi: 10.1128/JCM.42.3.1115-1122.2004.
5
Assessment of PCR-DGGE for the identification of diverse Helicobacter species, and application to faecal samples from zoo animals to determine Helicobacter prevalence.评估聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）用于鉴定多种幽门螺杆菌物种，并应用于动物园动物粪便样本以确定幽门螺杆菌的流行情况。
J Med Microbiol. 2003 Sep;52(Pt 9):765-771. doi: 10.1099/jmm.0.05314-0.
6
Evaluation of 11 PCR assays for species-level identification of Campylobacter jejuni and Campylobacter coli.用于空肠弯曲菌和结肠弯曲菌种水平鉴定的11种聚合酶链反应检测方法的评估。
J Clin Microbiol. 2003 Jan;41(1):330-6. doi: 10.1128/JCM.41.1.330-336.2003.
7
A group-specific PCR assay for the detection of Helicobacteraceae in human gut.一种用于检测人肠道中螺杆菌科细菌的组特异性聚合酶链反应检测方法。
Helicobacter. 2002 Dec;7(6):378-83. doi: 10.1046/j.1523-5378.2002.00113.x.
8
Identification of novel Helicobacter species in pig stomachs by PCR and partial sequencing.通过聚合酶链反应（PCR）和部分测序鉴定猪胃中的新型幽门螺杆菌菌种。
J Clin Microbiol. 2001 Sep;39(9):3311-5. doi: 10.1128/JCM.39.9.3311-3315.2001.
9
Identification of enterohepatic Helicobacter species by restriction fragment-length polymorphism analysis of the 16S rRNA gene.通过16S rRNA基因的限制性片段长度多态性分析鉴定肠肝螺杆菌属菌种
Helicobacter. 2000 Sep;5(3):121-8. doi: 10.1046/j.1523-5378.2000.00019.x.
10
Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in a human gastric biopsy.通过扩增16S rRNA基因检测幽门螺杆菌属以及在人胃活检中鉴定猫幽门螺杆菌的策略。
Res Microbiol. 1997 May;148(4):315-26. doi: 10.1016/S0923-2508(97)81587-2.

基于16S rRNA基因的聚合酶链反应检测法对幽门螺杆菌属水平鉴定的评估

Evaluation of 16S rRNA gene-based PCR assays for genus-level identification of Helicobacter species.

作者信息

Moyaert H, Pasmans F, Ducatelle R, Haesebrouck F, Baele M

机构信息

Department of Pathology, Bacteriology, and Avian Diseases, Ghent University, Faculty of Veterinary Medicine, Salisburylaan 133, B-9820 Merelbeke, Belgium.

出版信息

J Clin Microbiol. 2008 May;46(5):1867-9. doi: 10.1128/JCM.00139-08. Epub 2008 Mar 12.

DOI:10.1128/JCM.00139-08
PMID:18337392
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2395105/
Abstract

The inclusivity, exclusivity, and detection limit of six 16S rRNA gene-based Helicobacter genus-specific PCR assays were examined. Five out of six assays were 100% inclusive, but the tests varied considerably in their exclusivity (9.1 to 95.5%). The clinical detection limit varied between 10(3) and 1 viable bacterial cell per reaction mixture.

摘要

检测了六种基于16S rRNA基因的幽门螺杆菌属特异性PCR检测方法的包容性、排他性和检测限。六种检测方法中有五种具有100%的包容性，但这些检测在排他性方面差异很大（9.1%至95.5%）。临床检测限在每个反应混合物10³个至1个活细菌细胞之间变化。