Benson D A, Karsch-Mizrachi I, Lipman D J, Ostell J, Rapp B A, Wheeler D L
National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Building 38A, 8600 Rockville Pike, Bethesda, MD 20894, USA.
Nucleic Acids Res. 2000 Jan 1;28(1):15-8. doi: 10.1093/nar/28.1.15.
The GenBank((R))sequence database incorporates publicly available DNA sequences of >55 000 different organisms, primarily through direct submission of sequence data from individual laboratories and large-scale sequencing projects. Most submissions are made using the BankIt (Web) or Sequin programs and accession numbers are assigned by GenBank staff upon receipt. Data exchange with the EMBL Data Library and the DNA Data Bank of Japan helps ensure comprehensive worldwide coverage. GenBank data is accessible through NCBI's integrated retrieval system, Entrez, which integrates data from the major DNA and protein sequence databases along with taxonomy, genome, mapping and protein structure information, plus the biomedical literature via PubMed. Sequence similarity searching is provided by the BLAST family of programs. Complete bimonthly releases and daily updates of the GenBank database are available by FTP. NCBI also offers a wide range of WWW retrieval and analysis services based on GenBank data. The GenBank database and related resources are freely accessible via the NCBI home page at http://www.ncbi.nlm.nih.gov
GenBank((R))序列数据库收录了超过55000种不同生物的公开可用DNA序列，主要通过各个实验室直接提交序列数据以及大规模测序项目来实现。大多数提交是使用BankIt（网络版）或Sequin程序进行的，GenBank工作人员在收到数据后会分配登录号。与欧洲分子生物学实验室（EMBL）数据库和日本DNA数据库的数据交换有助于确保全球范围的全面覆盖。GenBank数据可通过美国国立医学图书馆（NCBI）的综合检索系统Entrez获取，该系统整合了来自主要DNA和蛋白质序列数据库的数据，以及分类学、基因组、图谱和蛋白质结构信息，还通过PubMed整合了生物医学文献。序列相似性搜索由BLAST系列程序提供。GenBank数据库每两个月进行一次完整发布，并通过FTP进行每日更新。NCBI还基于GenBank数据提供了广泛的万维网检索和分析服务。可通过NCBI主页（http://www.ncbi.nlm.nih.gov）免费访问GenBank数据库及相关资源。
