Escoula L
Ann Rech Vet. 1975;6(3):303-10.
The toxinogenesis of 18 strains of Byssochlamys nivea, 4 of Byssochlamys fulva, 7 of Paecilomyces varioti, 2 of Aspergillus clavatus, I of Aspergillus terreus, and I of Penicillium urticae, isolated in ensilaged forages, is tested at 26 degrees C in Czapek's enriched liquid medium (8 p. 1000 glucose + 2 p. 1000 yeast extract). 100 p. 100 of Byssochlamys nivea strains, 75 p. 100 of Byssochlamys fulva and 12 p. 100 of Paecilomyces varioti strains produce patulin after 9 days of culture. Mycotoxin concentrations recovered range from 0.5 to 1 120 p.p.m. Only highly toxinogenic strains when they invade silage cutting fronts, would be able to develop detectable amounts of patulin.
对从青贮饲料中分离出的18株雪白丝衣霉、4株黄丝衣霉、7株变幻青霉、2株棒曲霉、1株土曲霉和1株荨麻青霉进行产毒试验,在26℃的查氏富集液体培养基(每1000份含8份葡萄糖 + 2份酵母提取物)中培养。培养9天后,100%的雪白丝衣霉菌株、75%的黄丝衣霉菌株和12%的变幻青霉菌株产生展青霉素。回收的霉菌毒素浓度范围为0.5至1120 ppm。只有高产毒株在侵入青贮饲料切割面时,才能够产生可检测量的展青霉素。