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[同时测定流速、电解质和蛋白质浓度以及人腮腺唾液分级的圆盘电泳分离(作者译)]

[Simultaneous estimation of flow rates, electrolyte and proteinconcentration and discelectrophoretic separation of fractionated human parotid saliva(author's transl)].

作者信息

Eichner H, Münzel M, Bretzel G

出版信息

Laryngol Rhinol Otol (Stuttg). 1975 Jan;54(1):19-27.

PMID:123296
Abstract

Fractionated human parotid saliva from normal persons was separated by column-acryamid-gel discelectrophoresis. Flow rates, electrolyte-concentrations and total protein content of the stimulated and unstimulated parotid-secretion were determined simultaneously. Significant patterns of proteincomposition were found for stimulated and unstimulated parotid saliva. Most typical changes of protein bands were found in the anodal near gel region, within a group of four bands, called by us f, g, h and i. In the cathodal near gel region as well significant changes in the bands a, b, c and d were found; by glycoprotein staining these bands showed good staining.

摘要

来自正常人的分次腮腺唾液通过柱丙烯酰胺凝胶圆盘电泳进行分离。同时测定了刺激和未刺激腮腺分泌液的流速、电解质浓度和总蛋白含量。在刺激和未刺激的腮腺唾液中发现了蛋白质组成的显著模式。蛋白质条带最典型的变化出现在凝胶阳极附近区域,在一组四条带中,我们将其称为f、g、h和i。在凝胶阴极附近区域,a、b、c和d条带也出现了显著变化;通过糖蛋白染色,这些条带显示出良好的染色效果。

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