Schlesinger M, Chaouat M
Eur J Immunol. 1975 Jan;5(1):27-32. doi: 10.1002/eji.1830050107.
The exposure of murine peritoneal cells to anti-H-2 sera results in a diminished expression of H-2 antigen on the cell surface. Concomitant with this "H-2 modulation" the capacity of macrophages to bind sheep red blood cells coated with antibody and complement (EAC) was markedly diminished. In contrast, there was no change in the capacity of modulated macrophages to bind sheep red blood cells coated with antibody alone (EA). Antibodies to K end H-2 specificities were more effective in reducing the binding of EAC than antibodies to D end H-2 specificities. Exposure of peritoneal cells to O or Ly antisera had no effect on the formation of EAC rosettes. Exposure of peritoneal cells to anti-H-2 sera, under conditions which would not allow modulation of H-2 antigens, also prevented the reduction of EAC binding. Thus, the EAC receptors and H-2 antigenic specificities seem to be closely related on the surface of peritoneal cells, but constitute distinct cell surface structures. Preliminary evidence indicates that vinblastine, a microtubule depolymerizing agent, may disrupt the close association of EAC receptors and H-2 antigens. It is suggested that the association of EAC receptors and K end H-2 determinants on the membrane of macrophages may have implications for the regulation of the immune response by H-2-linked Ir genes.
将小鼠腹膜细胞暴露于抗H - 2血清会导致细胞表面H - 2抗原表达减少。伴随着这种“H - 2调节”，巨噬细胞结合被抗体和补体包被的绵羊红细胞（EAC）的能力显著降低。相比之下，经调节的巨噬细胞结合仅被抗体包被的绵羊红细胞（EA）的能力没有变化。针对K端H - 2特异性的抗体在减少EAC结合方面比针对D端H - 2特异性的抗体更有效。将腹膜细胞暴露于O或Ly抗血清对EAC玫瑰花结的形成没有影响。在不允许H - 2抗原调节的条件下，将腹膜细胞暴露于抗H - 2血清也可防止EAC结合的减少。因此，EAC受体和H - 2抗原特异性在腹膜细胞表面似乎密切相关，但构成不同的细胞表面结构。初步证据表明，长春花碱，一种微管解聚剂，可能会破坏EAC受体与H - 2抗原的紧密结合。有人提出，巨噬细胞膜上EAC受体与K端H - 2决定簇的结合可能对H - 2连锁Ir基因调节免疫反应有影响。
