对凝集素产生反应的脾T淋巴细胞亚群的抗原特性。
Antigenic properties of subsets of splenic T lymphocytes responding to lectins.
作者信息
Schlesinger M, Israel E, Gery I
出版信息
Immunology. 1976 Jun;30(6):865-72.
The aim of the present study was to characterize the antigenic properties of spleen cells responding in vitro to various mitogenic stimuli. Mouse spleen cells were treated with alloantibodies and C and then cultured with various concentrations of lectins. The exposure of spleen cells to various dilutions of either anti-theta serum and C or anti-Ly serum and C inhibited the response to optimal concentrations of PHA to a greater extent than the response to optimal concentrations of Con A. With the exception of the optimal lectin concentration, theta-antiserum and C had a stronger inhibitory effect on the response to Con A than to PHA. The exposure of spleen cells to H-2 antiserum and C inhibited their response to Con A to a greater extent than to PHA. The inhibitory activity of H-2 antiserum and C was inversely correlated with the dose of PHA used for stimulation. The lower the concentration of PHA used for stimulation the more effective was the inhibitory effect of the antiserum. The inhibitory effect of H-2 antiserum and C on the response to Con A was highest at optimal concentrations of the mitogen, and somewhat less pronounced above or below this concentration. The present study suggests that subsets of splenic T-cells that react to various concentrations of mitogens, differ in their theta- and H-2 alloantigenicity.
本研究的目的是表征体外对各种促有丝分裂刺激作出反应的脾细胞的抗原特性。用同种抗体和补体处理小鼠脾细胞，然后用不同浓度的凝集素进行培养。将脾细胞暴露于抗θ血清和补体或抗Ly血清和补体的各种稀释液中，与对最佳浓度的刀豆蛋白A（Con A）的反应相比，对最佳浓度的植物血凝素（PHA）的反应受到的抑制程度更大。除了最佳凝集素浓度外，θ抗血清和补体对Con A反应的抑制作用比对PHA反应的抑制作用更强。将脾细胞暴露于H-2抗血清和补体中，其对Con A反应的抑制程度比对PHA反应的抑制程度更大。H-2抗血清和补体的抑制活性与用于刺激的PHA剂量呈负相关。用于刺激的PHA浓度越低，抗血清的抑制作用就越有效。H-2抗血清和补体对Con A反应的抑制作用在促有丝分裂原的最佳浓度时最高，在该浓度以上或以下则不太明显。本研究表明，对不同浓度促有丝分裂原作出反应的脾T细胞亚群，在其θ和H-2同种抗原性方面存在差异。
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