Shikata T, Yoshizaki C, Okazaki K, Uzawa T, Matsushita H
Gastroenterol Jpn. 1975;10(3):199-207. doi: 10.1007/BF02776653.
The relationship between the electron microscopical structures and the immunofluorescent appearance of HB antigen found in the hepatocytes of patients with HB antigenemia was carefully studied, with the purpose to clarify the nature of those structures. The nuclei of hepatocytes, containing numerous virus-like particles, showed positive immunoflourescence by antiserum against the core of Dane particles. The areas in which filamentous structures were observed in the cisternae of endoplasmic reticulums and hyaloplasma, corresponded to orcein or aldehyde-fuchsin positive substances in the light microscope, whereas direct relationship between filamentous structures and HBs antigen was still obscure. Accordingly, it may be concluded that virus-like particles in the nucleus correpond to core and filamentous structures in the cytoplasma correlate with coat protein of hepatitis B virus.
对乙肝表面抗原血症患者肝细胞中发现的乙肝抗原的电子显微镜结构与免疫荧光表现之间的关系进行了仔细研究，目的是阐明这些结构的性质。含有大量病毒样颗粒的肝细胞核，通过抗丹氏颗粒核心的抗血清显示出阳性免疫荧光。在内质网池和透明质中观察到丝状结构的区域，在光学显微镜下对应于orcein或醛复红阳性物质，而丝状结构与乙肝表面抗原之间的直接关系仍不明确。因此，可以得出结论，细胞核中的病毒样颗粒对应于核心，细胞质中的丝状结构与乙肝病毒的衣壳蛋白相关。
