土生隐球菌MCN2对四氰合镍（II）的降解
Degradation of tetracyanonickelate (II) by Cryptococcus humicolus MCN2.
作者信息
Kwon Hyouk K, Woo Seung H, Park Jong M
机构信息
Department of Chemical Engineering, School of Environmental Science and Engineering, Pohang University of Science and Technology, San 31, Pohang 790-784, South Korea.
出版信息
FEMS Microbiol Lett. 2002 Sep 10;214(2):211-6. doi: 10.1111/j.1574-6968.2002.tb11349.x.
A new yeast strain capable of degrading free and metallocyanides was isolated from coke-plant wastewater. The isolated strain designated MCN2 was identified as Cryptococcus humicolus by 26S rDNA sequencing and phylogenetic analysis. During growth of the isolate with KCN as a sole nitrogen source, formamide and formic acid were found as transient intermediates by [(13)C]nuclear magnetic resonance analysis and ammonia accumulated as a final product in the culture medium. The strain MCN2 could degrade high concentrations of tetracyanonickelate (II) (K(2)Ni(CN)(4), TCN) up to 65 mM CN within 60 h when a sufficient amount of glucose was supplied as a carbon source. The maximal degradation rate of TCN was 2.5 mM CN h(-1) at the initial concentration of 51 mM CN.
从焦化厂废水中分离出一种能够降解游离氰化物和金属氰化物的新型酵母菌株。通过26S rDNA测序和系统发育分析，将分离得到的菌株命名为MCN2，并鉴定为土生隐球菌。在以KCN作为唯一氮源培养该菌株的过程中，通过[(13)C]核磁共振分析发现甲酰胺和甲酸为瞬时中间体，氨作为最终产物积累在培养基中。当提供足够量的葡萄糖作为碳源时，菌株MCN2能够在60小时内降解高达65 mM CN的高浓度四氰合镍(II)酸钾（K(2)Ni(CN)(4)，TCN）。在初始浓度为51 mM CN时，TCN的最大降解速率为2.5 mM CN h(-1)。
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