表达玻璃珠菌血红蛋白可增强氧化葡萄糖酸杆菌的细胞生长和二羟丙酮的生成。
Expression of Vitreoscilla hemoglobin enhances cell growth and dihydroxyacetone production in Gluconobacter oxydans.
机构信息
State Key Laboratory of Bioreactor Engineering, Institute of Newworld Biotechnology, East China University of Science and Technology, Shanghai, China.
出版信息
Curr Microbiol. 2010 Nov;61(5):370-5. doi: 10.1007/s00284-010-9621-6. Epub 2010 Apr 1.
Dihydroxyacetone (DHA) is an important ketose sugar, which is extensively used in the cosmetic, chemical, and pharmaceutical industries. DHA has been industrially produced by Gluconobacter oxydans with a high demand of oxygen. To improve the production of DHA, the gene vgb encoding Vitreoscilla hemoglobin (VHb) was successfully introduced into G. oxydans, where it was stably maintained, and expressed at about 76.0 nmol/g dry cell weight. Results indicated that the constitutively expressed VHb improved cell growth and DHA production in G. oxydans under different aeration conditions. Especially at low aeration rates, the VHb-expressing strain (VHb(+)) displayed 23.13% more biomass and 37.36% more DHA production than those of VHb-free strain (VHb(-)) after 32 h fermentation in bioreactors. In addition, oxygen uptake rate (OUR) was also increased in VHb(+) strain relative to the control strain during fermentation processes.
二羟丙酮（DHA）是一种重要的酮糖，广泛应用于化妆品、化工和制药行业。DHA 已被氧化葡萄糖酸杆菌通过大量需氧的方式工业化生产。为了提高 DHA 的产量，成功将编码血晶素（VHb）的基因 vgb 导入氧化葡萄糖酸杆菌，该基因在其中稳定存在，并以约 76.0 nmol/g 干细胞重量的水平表达。结果表明，在不同通气条件下，组成型表达的 VHb 提高了葡萄糖酸杆菌的细胞生长和 DHA 产量。特别是在低通气率下，与无 VHb 表达的菌株（VHb(-)）相比，VHb 表达菌株（VHb(+)）在生物反应器中发酵 32 小时后，生物量增加了 23.13%，DHA 产量增加了 37.36%。此外，与对照菌株相比，VHb(+)菌株在发酵过程中的耗氧速率（OUR）也有所提高。
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