牙齿萌出过程中大鼠牙周组织中MMP - 8和MMP - 13 mRNA的表达

Expression of MMP-8 and MMP-13 mRNAs in rat periodontium during tooth eruption.

作者信息

Tsubota M, Sasano Y, Takahashi I, Kagayama M, Shimauchi H

机构信息

Division of Periodontics and Endodontics, Tohoku University Graduate School of Dentistry, Sendai 980-8575, Japan.

出版信息

J Dent Res. 2002 Oct;81(10):673-8. doi: 10.1177/154405910208101004.

DOI:10.1177/154405910208101004

PMID:12351664

Abstract

The present study was designed to investigate mRNA expression of matrix metalloproteinase-8 (MMP-8) and MMP-13 in forming periodontium during tooth eruption in the rat. RT-PCR for the decalcified paraffin sections indicated expression of MMP-8 and MMP-13 in the periodontal tissues. In situ hydridization demonstrated expression of MMP-8 in osteoblasts, osteocytes, periodontal ligament cells, cementoblasts, and cementocytes along with collagen types I and III. In contrast, transcripts of MMP-13 were confined to a small population of osteoblasts and osteocytes in alveolar bone. The results suggested that MMP-8 may be involved in remodeling the periodontium during tooth eruption, and its expression may be coordinated with that of collagen types I and III, whereas the participation of MMP-13 may be rather limited.

摘要

本研究旨在调查大鼠牙齿萌出过程中形成期牙周组织中基质金属蛋白酶-8（MMP-8）和MMP-13的mRNA表达。对脱钙石蜡切片进行的逆转录聚合酶链反应（RT-PCR）表明，牙周组织中有MMP-8和MMP-13的表达。原位杂交显示，MMP-8在成骨细胞、骨细胞、牙周膜细胞、成牙骨质细胞和牙骨质细胞中表达，同时伴有I型和III型胶原。相比之下，MMP-13的转录本局限于牙槽骨中一小部分成骨细胞和骨细胞。结果表明，MMP-8可能参与牙齿萌出过程中牙周组织的重塑，其表达可能与I型和III型胶原的表达协调一致，而MMP-13的参与可能相当有限。