Fonseca Barbara Alves, de Oliveira Fernandes Thaís, de Moura Dalila Ferreira Silvano, Reis Caio Luiz Bittencourt, Küchler Erika Calvano, Baratto-Filho Flares, Antunes Leonardo Santos, Antunes Lívia Azeredo Alves
Posgraduate Program in Dentistry, Institute of Health Sciences, Fluminense Federal University, Nova Friburgo, RJ, Brazil.
School of Biomedicine, Institute of Health Sciences, Fluminense Federal University, Nova Friburgo, RJ, Brazil.
Biochem Genet. 2025 Jan 8. doi: 10.1007/s10528-024-11016-9.
To analyze whether the single-nucleotide polymorphisms (SNPs) in Matrix metalloproteinases 2, 3, and 9 (MMP2, MMP3, and MMP9), Tissue Inhibitor of Metalloproteinases 1 and 2 (TIMP1 and TIMP2), methionine synthase (MTR) and methionine synthase reductase (MTRR) influence delayed deciduous tooth eruption (DDTE). This cross-sectional study included 1060 biologic unrelated children (aged between 6 and 36 months) of both sexes, selected from 25 public schools in Nova Friburgo, Rio de Janeiro, Brazil. Oral examination was conducted and DDTE was defined by the absence of gingival eruption according to a chronology based on the Brazilian population. Genotyping of selected SNPs (rs243847, rs52261, rs17576, rs4898, rs7501477, rs1805087, and rs1801394) was performed using TaqMan real-time PCR with genomic DNA extracted from buccal cells. The association between genotypes and DDTE was evaluated using univariate and multivariate analyses (p < 0.05). A total of 224 children and caregivers were included after the eligibility criteria. The heterozygous genotype for the SNPs MTR (rs11805087) was associated with DDTE in both the univariate (p = 0.004) and multivariate (p < 0.001) codominant models, as well as in the univariate (p = 0.010) and multivariate (p = 0.001) recessive models. TIMP1 (rs4898) and MMP3 (rs522616) were associated with DDTE only in the univariate model (p < 0.05). The SNPs in MTR (rs11805087), MMP3 (rs522616) and TIMP (rs4898) genes are associated with DDTE. The factors affecting the chronology of deciduous tooth eruption has been insufficiently studied. This article brings novel knowledge regarding the role of genetics polymorphisms on timing variation of deciduous tooth eruption. Understanding the factors that impact tooth eruption is crucial for the fields of pediatric dentistry and orthodontics.
分析基质金属蛋白酶2、3和9（MMP2、MMP3和MMP9）、金属蛋白酶组织抑制剂1和2（TIMP1和TIMP2）、甲硫氨酸合成酶（MTR）和甲硫氨酸合成酶还原酶（MTRR）中的单核苷酸多态性（SNP）是否会影响乳牙萌出延迟（DDTE）。这项横断面研究纳入了1060名来自巴西里约热内卢新弗里堡25所公立学校的6至36个月大的无生物学亲缘关系的儿童。进行了口腔检查，并根据基于巴西人群的时间顺序，将乳牙萌出延迟定义为牙龈未萌出。使用TaqMan实时PCR对从颊细胞中提取的基因组DNA进行选定SNP（rs243847、rs52261、rs17576、rs4898、rs7501477、rs1805087和rs1801394）的基因分型。使用单因素和多因素分析评估基因型与乳牙萌出延迟之间的关联（p < 0.05）。在符合纳入标准后，共纳入了224名儿童及其照料者。SNP MTR（rs11805087）的杂合基因型在单因素（p = 0.004）和多因素（p < 0.001）共显性模型以及单因素（p = 0.010）和多因素（p = 0.001）隐性模型中均与乳牙萌出延迟相关。TIMP1（rs4898）和MMP3（rs522616）仅在单因素模型中与乳牙萌出延迟相关（p < 0.05）。MTR（rs11805087）、MMP3（rs522616）和TIMP（rs4898）基因中的SNP与乳牙萌出延迟相关。影响乳牙萌出时间顺序的因素尚未得到充分研究。本文带来了关于基因多态性在乳牙萌出时间变化中作用的新知识。了解影响牙齿萌出的因素对于儿童牙科和正畸领域至关重要。
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