Schröder Agnes, Gubernator Joshua, Nazet Ute, Spanier Gerrit, Jantsch Jonathan, Proff Peter, Kirschneck Christian
Department of Orthodontics, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany.
Department of Cranio-Maxillo-Facial Surgery, University Hospital Regensburg, Franz-Josef-Strauß-Allee 11, 93053, Regensburg, Germany.
J Orofac Orthop. 2020 Sep;81(5):360-370. doi: 10.1007/s00056-020-00232-8. Epub 2020 Jul 6.
During orthodontic tooth movement, pressure and tension zones develop in the periodontal ligament, and periodontal ligament fibroblasts (PDLF) become exposed to mechanical strain. Enhanced salt (NaCl) concentrations are known to modulate responses of PDLF and immune cells to different stimuli like mechanical strain. Here, we investigated the impact of tensile strain on the gene expression profile of PDLF under normal (NS) and high salt (HS) conditions.
After preincubation under NS or HS (+40 mM NaCl in medium) conditions for 24 h, PDLF were stretched 16% for 48 h using custom-made spherical cap silicone stamps using an established and published setup. After determination of cell number and cytotoxicity, we analyzed expression of genes involved in extracellular matrix reorganization, angiogenesis, bone remodeling, and inflammation by quantitative real-time polymerase chain reaction (RT-qPCR).
Tensile strain did not affect the expression of genes involved in angiogenesis or extracellular matrix reorganization by PDLF, which however modulate inflammatory responses and bone remodeling in reaction to 16% static tensile strain. Salt (NaCl) treatment triggered enhanced extracellular matrix formation, expression of cyclooxygenase 2 and bone metabolism in PDLF during tensile strain.
Salt (NaCl) consumption may influence orthodontic tooth movement and periodontal bone loss via modulation of extracellular matrix and bone metabolism. Excessive salt intake during orthodontic therapy may cause adverse effects regarding periodontal inflammation and bone resorption.
在正畸牙齿移动过程中，牙周膜会形成压力区和张力区，牙周膜成纤维细胞（PDLF）会受到机械应变。已知盐（NaCl）浓度升高可调节PDLF和免疫细胞对不同刺激（如机械应变）的反应。在此，我们研究了在正常（NS）和高盐（HS）条件下拉伸应变对PDLF基因表达谱的影响。
在NS或HS（培养基中添加40 mM NaCl）条件下预孵育24小时后，使用定制的球形帽硅胶印章，按照既定且已发表的设置将PDLF拉伸16%，持续48小时。在确定细胞数量和细胞毒性后，我们通过定量实时聚合酶链反应（RT-qPCR）分析参与细胞外基质重组、血管生成、骨重塑和炎症的基因表达。
拉伸应变不影响PDLF中参与血管生成或细胞外基质重组的基因表达，但这些基因可调节对16%静态拉伸应变的炎症反应和骨重塑。盐（NaCl）处理在拉伸应变期间引发PDLF中细胞外基质形成增强、环氧合酶2表达和骨代谢。
盐（NaCl）摄入可能通过调节细胞外基质和骨代谢影响正畸牙齿移动和牙周骨丧失。正畸治疗期间过量摄入盐可能会对牙周炎症和骨吸收产生不利影响。
