利用聚合酶链式反应-短串联重复序列（PCR-STR）分析鉴定出疑似在水中浸泡三年的骨骼遗骸。

Skeletal remains presumed submerged in water for three years identified using PCR-STR analysis.

作者信息

Crainic K, Paraire F, Leterreux M, Durigon M, de Mazancourt P

机构信息

Laboratoire de Biochimie et Biologie Moléculaire, Hôpital R Poincaré, et Faculté de Médecine Paris-Quest, Garches, France.

出版信息

J Forensic Sci. 2002 Sep;47(5):1025-7.

PMID:12353539

Abstract

We describe the successful identification of the remains of a saponified body found in a dam by typing of nuclear DNA. Whereas DNA extracted from soft tissues yielded negative PCR results, DNA extracted from the bone by a slightly modified Qiagen procedure allowed the typing of sex (AMG locus) and of 10 additional STR loci. An identity document was found belonging to a man missing for 3 years and comparison of the results to the DNA profiles of his son and wife confirmed the identity. The longest delay reported until now for successful nuclear DNA genotyping after immersion in river water was 18 months. This case demonstrates a delay of up to 3 years.

摘要

我们描述了通过核DNA分型成功鉴定在大坝中发现的一具皂化尸体遗骸的过程。从软组织中提取的DNA进行PCR检测结果为阴性，而通过对Qiagen方法稍作修改后从骨骼中提取的DNA，能够进行性别（AMG基因座）及另外10个STR基因座的分型。发现了一份属于一名失踪3年男子的身份证件，将检测结果与他儿子和妻子的DNA图谱进行比对后确认了身份。此前报道的尸体浸入河水中后成功进行核DNA基因分型的最长延迟时间为18个月。本案显示延迟时间长达3年。