Sørensen Erik, Hansen Steen Holger, Eriksen Birthe, Morling Niels
Department of Forensic Genetics Institute of Forensic Medicine, University of Copenhagen, Frederik V's Vej 11, 2100, Copenhagen, Denmark.
Int J Legal Med. 2003 Aug;117(4):245-7. doi: 10.1007/s00414-003-0375-6. Epub 2003 Jun 19.
PCR amplification of DNA from forensic samples often proves difficult due to the presence of inhibitors of the polymerase chain reaction. One possible way to remove PCR inhibitors from a DNA extract is the use of the affinity resin thiopropyl sepharose 6B (TS), which has been used previously for the removal of PCR inhibitors in DNA extracts originating from stains on clothing. Here we show that TS is efficient also for the removal of inhibitors from PCR extracts from a highly decomposed human thigh bone. TS treatment, however, leads to a substantial loss of DNA making the technique best suited when substantial amounts of DNA are present.
由于存在聚合酶链反应抑制剂，从法医样本中进行DNA的PCR扩增往往很困难。从DNA提取物中去除PCR抑制剂的一种可能方法是使用亲和树脂硫丙基琼脂糖6B（TS），该树脂先前已用于去除源自衣物污渍的DNA提取物中的PCR抑制剂。在此我们表明，TS对于从高度腐烂的人股骨的PCR提取物中去除抑制剂也很有效。然而，TS处理会导致DNA大量损失，这使得该技术最适合于存在大量DNA的情况。
