基于两例碎尸案探讨血管作为基于聚合酶链反应的基因分型DNA来源的实用性。

Usefulness of blood vessels as a DNA source for PCR-based genotyping based on two cases of corpse dismemberment.

作者信息

Shintani-Ishida Kaori, Harada Kazuki, Nakajima Makoto, Yoshida Ken-ichi

机构信息

Department of Forensic Medicine, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, 113-0033 Tokyo, Japan.

出版信息

Leg Med (Tokyo). 2010 Jan;12(1):8-12. doi: 10.1016/j.legalmed.2009.09.004. Epub 2009 Oct 22.

DOI:10.1016/j.legalmed.2009.09.004

PMID:19853489

Abstract

The success of PCR-based genotyping of decomposed remains depends on the quality of extracted DNA. Hard tissues and muscles are preferred because of their DNA stability. However, in dismembered corpses the choice of a suitable DNA source is more limited. In short tandem repeat (STR) analysis in two cases of dismembered corpses, we found an advantage of using blood vessels over muscles. To confirm that blood vessels are better for STR typing compared to muscle, we collected nine sets of blood vessels and the adjacent muscle from six other decomposed remains and compared the STR profiles between the blood vessel and muscle samples. Better results for STR typing were obtained in blood vessels. Based on these results, we recommend use of blood vessels as material for PCR-based genotyping in identification of dismembered human remains with heavy postmortem changes.

摘要

基于聚合酶链反应（PCR）的腐败遗骸基因分型的成功与否取决于提取的DNA质量。由于硬组织和肌肉中的DNA稳定性较好，因此优先选择它们作为DNA来源。然而，在碎尸案件中，合适的DNA来源选择更为有限。在两起碎尸案件的短串联重复序列（STR）分析中，我们发现使用血管比使用肌肉具有优势。为了证实与肌肉相比，血管更适合进行STR分型，我们从另外六具腐败遗骸中收集了九组血管及其相邻肌肉，并比较了血管和肌肉样本之间的STR图谱。结果显示，血管样本的STR分型效果更好。基于这些结果，我们建议在鉴定有严重死后变化的碎尸时，使用血管作为基于PCR的基因分型材料。