Greineder D K, Rosenthal A S
J Immunol. 1975 May;114(5):1541-7.
The role of the macrophage in the guinea pig mixed leukocyte culture was investigated. Macrophages obtained from oil-induced peritoneal exudates, peritoneal wash-out cells, spleen, and alveolar washings were found to be effective stimulators of allogeneic lymph node and splenic lymphocyte DNA synthesis. The stimulatory properties of macrophages proved radioresistant but viability dependent. Unfractionated lymph node cells or adherence column purified lymph node lymphocytes and thymocytes were only minimally active as stimulators, even in the presence of macrophages syngeneic to the responder lymphocytes. Allogeneic fibroblasts, polymorphonuclear leukocytes, L2C leukemia cells, and xenogeneic (murine) macrophages failed to simulate. These data provide evidence that the macrophage is the predominant stimulator of the mixed leukocyte culture in the guinea pig.
对巨噬细胞在豚鼠混合白细胞培养中的作用进行了研究。从油诱导的腹腔渗出液、腹腔冲洗细胞、脾脏和肺泡灌洗中获得的巨噬细胞被发现是同种异体淋巴结和脾淋巴细胞DNA合成的有效刺激物。巨噬细胞的刺激特性经证实具有抗辐射性，但依赖于活力。未分级的淋巴结细胞或经黏附柱纯化的淋巴结淋巴细胞和胸腺细胞作为刺激物的活性极低，即使在存在与反应淋巴细胞同基因的巨噬细胞的情况下也是如此。同种异体成纤维细胞、多形核白细胞、L2C白血病细胞和异种（鼠）巨噬细胞均无法起到刺激作用。这些数据证明巨噬细胞是豚鼠混合白细胞培养中的主要刺激物。
