Holt P G
Immunology. 1979 Jun;37(2):429-36.
Macrophages were prepared from the lung, peritoneal cavity and blood of normal, unstimulated rats from a number of strains. The macrophages were purified by adherence, and characterized via surface markers, enzyme activity and phagocytic capacity, and subsequently tested for activity in cultures of mitogen-stimulated syngeneic lymphocytes. Peritoneal macrophages and blood monocytes were mildly stimulatory, or ineffective in modulating mitogen-induced DNA synthesis; peritoneal macrophages reconstituted the blastogenic responses of macrophage-depleted lymph node cell cultures to normal limits. In contrast, alveolar macrophages were markedly inhibitory to lymphocyte proliferation; in some instances inhibitory activity was demonstrable when added alveolar macrophages comprised only 0.04% of the total cells in culture. Lymphocyte proliferation induced by T-cell mitogens was more susceptible to this inhibition than was proliferation induced by the B-cell mitogen LPS. Alveolar macrophages recovered from SPF rats, while less in number, exhibited comparable inhibitory activity. These results form part of an emerging picture picture of the normal alveolar macrophage as a potential 'suppressor' of T-cell activity in the lung.
从多个品系的正常、未受刺激大鼠的肺、腹腔和血液中制备巨噬细胞。通过贴壁法纯化巨噬细胞，并通过表面标志物、酶活性和吞噬能力进行表征，随后在丝裂原刺激的同基因淋巴细胞培养物中测试其活性。腹腔巨噬细胞和血液单核细胞具有轻度刺激作用，或在调节丝裂原诱导的DNA合成方面无效；腹腔巨噬细胞可将巨噬细胞耗竭的淋巴结细胞培养物的增殖反应恢复到正常水平。相比之下，肺泡巨噬细胞对淋巴细胞增殖具有明显的抑制作用；在某些情况下，当添加的肺泡巨噬细胞仅占培养物中总细胞的0.04%时，抑制活性就很明显。T细胞丝裂原诱导的淋巴细胞增殖比B细胞丝裂原LPS诱导的增殖更容易受到这种抑制。从无特定病原体（SPF）大鼠中回收的肺泡巨噬细胞数量较少，但表现出相当的抑制活性。这些结果构成了正常肺泡巨噬细胞作为肺中T细胞活性潜在“抑制因子”这一新兴图景的一部分。
