Moreno-Fierros Leticia, Pérez-Ordóñez Inés, Palomar-Morales Martín
Unidad de Morfologi;a y Función, FES-Iztacala-UNAM, Ap. postal 314, Tlalnepantla, Méx., Mexico.
Life Sci. 2002 Oct 18;71(22):2667-80. doi: 10.1016/s0024-3205(02)02100-8.
Since the immune response appears to be variable according to the hormonal stage of the mammalian female, the aim of this study was to determine whether estrous cycle stage modifies the mucosal and systemic immune responses induced by intraperitoneal and vaginal immunization of mice with protoxin Cry1Ac. We tested the influence of three immunizations on the specific antibody response elicited at estrus and diestrus, that were the same estrous cycle stages at which the antigen was applied. Both intraperitoneal and vaginal immunization of mice with Cry1Ac either at estrus or diestrus induces specific antibody responses at serum, vagina and large intestine. The stage of the estrous cycle have little or non influence in the magnitude of the response induced, since only at serum the IgM was slightly higher at estrus than at diestrus by both routes. At the large intestine only the IgA response elicited via the intraperitoneal route changed, being higher at diestrus, whereas at the vagina IgA response induced did not change significantly due to the cycle stage. Present results suggest that Cry1Ac may be used as an antigen carrier as it can elicit antibody responses at systemic level and at several mucosal sites including the vagina that are not modified significantly throughout the reproductive cycle.
由于免疫反应似乎会根据哺乳动物雌性的激素阶段而变化，本研究的目的是确定发情周期阶段是否会改变用原毒素Cry1Ac对小鼠进行腹腔内和阴道免疫诱导的黏膜和全身免疫反应。我们测试了三次免疫对在发情期和间情期引发的特异性抗体反应的影响，这两个时期是应用抗原时相同的发情周期阶段。在发情期或间情期用Cry1Ac对小鼠进行腹腔内和阴道免疫，均可在血清、阴道和大肠中诱导特异性抗体反应。发情周期阶段对诱导反应的强度影响很小或没有影响，因为仅在血清中，两种途径下发情期的IgM均略高于间情期。在大肠中，仅通过腹腔途径引发的IgA反应发生了变化，间情期更高，而在阴道中，诱导的IgA反应并未因周期阶段而发生显著变化。目前的结果表明，Cry1Ac可作为抗原载体，因为它能在全身水平以及包括阴道在内的几个黏膜部位引发抗体反应，且在整个生殖周期中这些反应不会发生显著改变。
