Vázquez-Padrón R I, Moreno-Fierros L, Neri-Bazán L, Martínez-Gil A F, de-la-Riva G A, López-Revilla R
Center for Genetic Engineering and Biotechnology, Havana, Cuba.
Braz J Med Biol Res. 2000 Feb;33(2):147-55. doi: 10.1590/s0100-879x2000000200002.
The present paper describes important features of the immune response induced by the Cry1Ac protein from Bacillus thuringiensis in mice. The kinetics of induction of serum and mucosal antibodies showed an immediate production of anti-Cry1Ac IgM and IgG antibodies in serum after the first immunization with the protoxin by either the intraperitoneal or intragastric route. The antibody fraction in serum and intestinal fluids consisted mainly of IgG1. In addition, plasma cells producing anti-Cry1Ac IgG antibodies in Peyer's patches were observed using the solid-phase enzyme-linked immunospot (ELISPOT). Cry1Ac toxin administration induced a strong immune response in serum but in the small intestinal fluids only anti-Cry1Ac IgA antibodies were detected. The data obtained in the present study confirm that the Cry1Ac protoxin is a potent immunogen able to induce a specific immune response in the mucosal tissue, which has not been observed in response to most other proteins.
本文描述了苏云金芽孢杆菌Cry1Ac蛋白在小鼠体内诱导的免疫反应的重要特征。血清和黏膜抗体诱导的动力学表明，首次通过腹腔内或胃内途径用原毒素免疫后，血清中立即产生抗Cry1Ac IgM和IgG抗体。血清和肠液中的抗体成分主要为IgG1。此外，使用固相酶联免疫斑点法（ELISPOT）观察到派尔集合淋巴结中产生抗Cry1Ac IgG抗体的浆细胞。给予Cry1Ac毒素可在血清中诱导强烈的免疫反应，但在小肠液中仅检测到抗Cry1Ac IgA抗体。本研究获得的数据证实，Cry1Ac原毒素是一种有效的免疫原，能够在黏膜组织中诱导特异性免疫反应，而对大多数其他蛋白质的反应中未观察到这种情况。
