Moreno-Fierros L, García N, Gutiérrez R, López-Revilla R, Vázquez-Padrón R I
Unidad de Morfología y Función, ENEP-Iztacala-UNAM, Tlalnepantla, Mexico.
Microbes Infect. 2000 Jul;2(8):885-90. doi: 10.1016/s1286-4579(00)00398-1.
Recently we discovered that the Cry1Ac protoxin of Bacillus thuringiensis administered to Balb/c mice intraperitoneally (i.p.) or intragastrically is a systemic and intestinal immunogen as potent as cholera toxin. To further characterize the mucosal immunogenicity of Cry1Ac we additionally tried the intranasal (i.n.) and rectal routes and used enzyme-linked immunoassays to determine anti-Cry1Ac antibody responses in the serum as well as in vaginal and tracheobronchial washes and in the fluids of the large and the small intestine. Immunization by the i.p., i.n. and rectal routes induced IgM, IgG and IgA antibodies in all the mucosal surfaces analyzed, but the magnitude and predominant isotype of each response depended on the route used and the mucosal site analyzed. These data extend our findings on the striking mucosal immunogencity of Cry1Ac and provide additional evidence on the compartmentalization of the mucosal immune system.
最近我们发现，将苏云金芽孢杆菌的Cry1Ac原毒素经腹腔内（i.p.）或胃内给予Balb/c小鼠时，它是一种全身性和肠道免疫原，其效力与霍乱毒素相当。为了进一步表征Cry1Ac的黏膜免疫原性，我们还尝试了鼻内（i.n.）和直肠途径，并使用酶联免疫测定法来确定血清、阴道和气管支气管灌洗液以及大肠和小肠液中的抗Cry1Ac抗体反应。通过腹腔内、鼻内和直肠途径进行免疫，在所有分析的黏膜表面均诱导出IgM、IgG和IgA抗体，但每种反应的强度和主要同种型取决于所采用的途径和所分析的黏膜部位。这些数据扩展了我们关于Cry1Ac显著黏膜免疫原性的发现，并为黏膜免疫系统的区室化提供了更多证据。
