使用16S rRNA基因测序鉴定从单一囊性纤维化中心就诊患者中分离出的非发酵革兰氏阴性杆菌。
Use of 16S rRNA gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center.
作者信息
Ferroni Agnes, Sermet-Gaudelus Isabelle, Abachin Eric, Quesne Gilles, Lenoir Gerard, Berche Patrick, Gaillard Jean-Louis
机构信息
Microbiology Laboratory, Hôpital Necker-Enfants Malades, 149 rue de Sèvres, 75015 Paris, France.
出版信息
J Clin Microbiol. 2002 Oct;40(10):3793-7. doi: 10.1128/JCM.40.10.3793-3797.2002.
Abstract
During 1999, we used partial 16S rRNA gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. Of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. These 46 isolates were genotypically identified as Pseudomonas aeruginosa (19 isolates, 12 patients), Achromobacter xylosoxidans (10 isolates, 8 patients), Stenotrophomonas maltophilia (9 isolates, 9 patients), Burkholderia cepacia genomovar I/III (3 isolates, 3 patients), Burkholderia vietnamiensis (1 isolate), Burkholderia gladioli (1 isolate), and Ralstonia mannitolilytica (3 isolates, 2 patients), a recently recognized species.
摘要
1999年期间，我们运用部分16S rRNA基因测序技术对从我院囊性纤维化中心就诊患者中分离出的非典型非发酵革兰氏阴性杆菌进行前瞻性鉴定。从148例患者中分离出1093株非发酵革兰氏阴性杆菌，其中46株（4.2%）经传统表型试验得出的结果存在问题。这46株菌株经基因分型鉴定为铜绿假单胞菌（19株，12例患者）、木糖氧化无色杆菌（10株，8例患者）、嗜麦芽窄食单胞菌（9株，9例患者）、洋葱伯克霍尔德菌基因I/III型（3株，3例患者）、越南伯克霍尔德菌（1株）、唐菖蒲伯克霍尔德菌（1株）以及嗜甘露醇罗尔斯顿菌（3株，2例患者），嗜甘露醇罗尔斯顿菌是一种最近才被确认的菌种。
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