Buckner B., Kelson T. L., Robertson D. S.
Department of Genetics, Iowa State University, Ames, Iowa 50011.
Plant Cell. 1990 Sep;2(9):867-876. doi: 10.1105/tpc.2.9.867.
The y1 gene is one of the genes responsible for the production of [beta]-carotene in the endosperm and leaves of maize. We have cloned a Robertson's Mutator-tagged allele of the y1 gene (y1-mum) by using a Mu3 element as a hybridization probe. We substantiate that the cloned sequence is a portion of the y1 gene by molecular analyses of a revertant of a putative Mutator-induced y1 allele and the incidence of insertions within the cloned y1 sequence from several independently derived Mutator-induced y1 mutant stocks. The y1-mum sequence was used to isolate the standard Y1 allele, which conditions the presence of [beta]-carotene in the endosperm of the maize kernel.
y1基因是负责在玉米胚乳和叶片中产生β-胡萝卜素的基因之一。我们通过使用Mu3元件作为杂交探针,克隆了y1基因的一个罗伯逊突变体标签等位基因(y1-mum)。通过对一个假定的Mutator诱导的y1等位基因的回复体进行分子分析,以及对来自几个独立衍生的Mutator诱导的y1突变体库的克隆y1序列内插入事件的发生率分析,我们证实克隆的序列是y1基因的一部分。y1-mum序列被用于分离标准Y1等位基因,该等位基因决定了玉米籽粒胚乳中β-胡萝卜素的存在。