Suppr超能文献

玉米y1基因座的克隆,一个参与类胡萝卜素生物合成的基因。

Cloning of the y1 Locus of Maize, a Gene Involved in the Biosynthesis of Carotenoids.

作者信息

Buckner B., Kelson T. L., Robertson D. S.

机构信息

Department of Genetics, Iowa State University, Ames, Iowa 50011.

出版信息

Plant Cell. 1990 Sep;2(9):867-876. doi: 10.1105/tpc.2.9.867.

Abstract

The y1 gene is one of the genes responsible for the production of [beta]-carotene in the endosperm and leaves of maize. We have cloned a Robertson's Mutator-tagged allele of the y1 gene (y1-mum) by using a Mu3 element as a hybridization probe. We substantiate that the cloned sequence is a portion of the y1 gene by molecular analyses of a revertant of a putative Mutator-induced y1 allele and the incidence of insertions within the cloned y1 sequence from several independently derived Mutator-induced y1 mutant stocks. The y1-mum sequence was used to isolate the standard Y1 allele, which conditions the presence of [beta]-carotene in the endosperm of the maize kernel.

摘要

y1基因是负责在玉米胚乳和叶片中产生β-胡萝卜素的基因之一。我们通过使用Mu3元件作为杂交探针,克隆了y1基因的一个罗伯逊突变体标签等位基因(y1-mum)。通过对一个假定的Mutator诱导的y1等位基因的回复体进行分子分析,以及对来自几个独立衍生的Mutator诱导的y1突变体库的克隆y1序列内插入事件的发生率分析,我们证实克隆的序列是y1基因的一部分。y1-mum序列被用于分离标准Y1等位基因,该等位基因决定了玉米籽粒胚乳中β-胡萝卜素的存在。

相似文献

引用本文的文献

本文引用的文献

7
Nucleotide sequence of the maize transposable element Mul.玉米转座因子Mul的核苷酸序列。
Nucleic Acids Res. 1984 Aug 10;12(15):5955-67. doi: 10.1093/nar/12.15.5955.
8
Molecular analysis of the maize anthocyanin regulatory locus C1.玉米花青素调控位点C1的分子分析
Proc Natl Acad Sci U S A. 1986 Dec;83(24):9631-5. doi: 10.1073/pnas.83.24.9631.

文献检索

告别复杂PubMed语法,用中文像聊天一样搜索,搜遍4000万医学文献。AI智能推荐,让科研检索更轻松。

立即免费搜索

文件翻译

保留排版,准确专业,支持PDF/Word/PPT等文件格式,支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述,25分钟生成高质量综述,智能提取关键信息,辅助科研写作。

立即免费体验