Ng T B, Parkash A, Tso W W
Department of Biochemistry, Faculty of Medicine, Basic Medical Sciences Bldg. Rm. 302B, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, China.
Protein Expr Purif. 2002 Oct;26(1):9-13. doi: 10.1016/s1046-5928(02)00500-4.
From fresh brown pumpkin seeds, two proteins with a molecular mass of 12kDa and an N-terminal sequence rich in arginine and glutamate residues were obtained. The protein designated alpha-moschin closely resembled the fruitfly programmed-cell death gene product and the protein designated beta-moschin demonstrated striking similarity to prepro 2S albumin in N-terminal sequence. alpha- and beta-moschins inhibited translation in the rabbit reticulocyte lysate system with an IC(50) of 17 microM and 300nM, respectively.
从新鲜的棕色南瓜籽中获得了两种分子量为12kDa且N端序列富含精氨酸和谷氨酸残基的蛋白质。命名为α-南瓜蛋白的蛋白质与果蝇程序性细胞死亡基因产物非常相似，命名为β-南瓜蛋白的蛋白质在N端序列上与前体2S白蛋白表现出显著相似性。α-和β-南瓜蛋白在兔网织红细胞裂解物系统中抑制翻译，IC(50)分别为17微摩尔和300纳摩尔。
