Wang H X, Ng T B
Department of Microbiology, College of Biological Science, China Agricultural University, Beijing, China.
Peptides. 2003 Jul;24(7):969-72. doi: 10.1016/s0196-9781(03)00191-8.
A novel antifungal peptide, with a molecular mass of 8 kDa in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and in gel filtration on Superdex 75 and designated cucurmoschin, was isolated from the seeds of the black pumpkin. The peptide was unadsorbed on DEAE-cellulose but adsorbed on Affi-gel blue gel. Cucurmoschin inhibited mycelial growth in the fungi Botrytis cinerea, Fusarium oxysporum and Mycosphaerella oxysporum. It inhibited translation in a cell-free rabbit reticulocyte lysate system with an IC50 of 1.2 microM. The N-terminal sequence of cucurmoschin was rich in arginine, glutamate and glycine residues.
从黑南瓜种子中分离出一种新型抗真菌肽，在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳中分子量为8 kDa，在Superdex 75凝胶过滤中也为8 kDa，命名为南瓜抗菌肽。该肽不吸附于DEAE-纤维素，但吸附于Affi-凝胶蓝胶。南瓜抗菌肽可抑制灰葡萄孢菌、尖孢镰刀菌和尖孢小球腔菌的菌丝生长。在无细胞兔网织红细胞裂解物系统中，它可抑制翻译，IC50为1.2 microM。南瓜抗菌肽的N端序列富含精氨酸、谷氨酸和甘氨酸残基。
