从裙带菜（海带）中提取的血管紧张素I转换酶抑制肽及其对自发性高血压大鼠的降压作用。

Angiotensin I-converting enzyme inhibitory peptides derived from wakame (Undaria pinnatifida) and their antihypertensive effect in spontaneously hypertensive rats.

作者信息

Sato Minoru, Hosokawa Takao, Yamaguchi Toshiyasu, Nakano Toshiki, Muramoto Koji, Kahara Takashi, Funayama Katsura, Kobayashi Akio, Nakano Takahisa

机构信息

Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidoriamamiyamachi, Aoba-ku, Sendai, Miyagi, 981-8555, Japan.

出版信息

J Agric Food Chem. 2002 Oct 9;50(21):6245-52. doi: 10.1021/jf020482t.

DOI:10.1021/jf020482t

PMID:12358510

Abstract

Seven kinds of angiotensin I-converting enzyme (ACE) inhibitory peptides were isolated from the hydrolysates of wakame (Undaria pinnatifida) by Protease S "Amano" (from Bacillus stearothermophilus) by using three-step high-performance liquid chromatography (HPLC) on a reverse-phase column. These peptides were identified by amino acid composition analysis, sequence analysis, and liquid chromatography-mass spectrometry (LC-MS), as Val-Tyr (IC(50) = 35.2 microM), Ile-Tyr (6.1 microM), Ala-Trp (18.8 microM), Phe-Tyr (42.3 microM), Val-Trp (3.3 microM), Ile-Trp (1.5 microM), and Leu-Trp (23.6 microM). These peptides have resistance against gastrointestinal proteases in vitro. Each peptide was determined to have an antihypertensive effect after a single oral administration in spontaneously hypertensive rats (SHR). Among them, the blood pressure significantly decreased by Val-Tyr, Ile-Tyr, Phe-Tyr, and Ile-Trp in a dose of 1 mg/kg of body weight (BW). The present study showed that antihypertensive effect in the hydrolysates of wakame by Protease S "Amano" was attributed to these peptides.

摘要

通过使用三步高效液相色谱法（HPLC）在反相柱上，从蛋白酶S“天野”（来自嗜热脂肪芽孢杆菌）水解的裙带菜（海带）水解物中分离出七种血管紧张素I转换酶（ACE）抑制肽。通过氨基酸组成分析、序列分析和液相色谱-质谱联用（LC-MS）鉴定这些肽为Val-Tyr（IC50 = 35.2 microM）、Ile-Tyr（6.1 microM）、Ala-Trp（18.8 microM）、Phe-Tyr（42.3 microM）、Val-Trp（3.3 microM）、Ile-Trp（1.5 microM）和Leu-Trp（23.6 microM）。这些肽在体外对胃肠道蛋白酶具有抗性。在自发性高血压大鼠（SHR）中单次口服给药后，确定每种肽都具有降压作用。其中，剂量为1 mg/kg体重（BW）时，Val-Tyr、Ile-Tyr、Phe-Tyr和Ile-Trp可使血压显著降低。本研究表明，蛋白酶S“天野”水解的裙带菜水解物中的降压作用归因于这些肽。