Santos I M, Abrunhosa L, Venâncio A, Lima N
Micoteca da Universidade do Minho and Centro de Engenharia Biológica da Universidade do Minho, Braga, Portugal.
Lett Appl Microbiol. 2002;35(4):272-5. doi: 10.1046/j.1472-765x.2002.01186.x.
To study the influence of culture preservation methods and culture conditions on the production of the mycotoxins patulin and citrinin by Penicillium expansum.
Ten strains of Penicillium expansum were preserved using subculture and maintenance at 4 degrees C, mineral oil, drying on silica gel and freeze-drying. Patulin and citrinin production was assessed on yeast extract sucrose agar (YES) and grape juice agar (GJ), using TLC before and after 0.5, 2-3, 6 and 12 months preservation. Citrinin was detected in all cultures for all preservation techniques on YES. The patulin profiles obtained differed with strain and culture media used.
Citrinin production seems to be a stable character for the tested strains. There is a tendency for patulin detection with time apparently more consistent for silica gel storage and freeze-drying, especially when the strains are grown on GJ.
Variability in the profiles of the mycotoxins tested seems to be more strain-specific than dependent on the preservation technique used.
研究保藏方法和培养条件对扩展青霉产生棒曲霉素和桔霉素这两种霉菌毒素的影响。
采用传代培养并于4℃保存、矿物油、硅胶干燥和冷冻干燥等方法对10株扩展青霉进行保藏。在酵母提取物蔗糖琼脂（YES）和葡萄汁琼脂（GJ）上评估棒曲霉素和桔霉素的产生情况，在保藏0.5、2 - 3、6和12个月前后使用薄层色谱法进行检测。在YES上，所有保藏技术的所有培养物中均检测到桔霉素。所获得的棒曲霉素图谱因菌株和所用培养基的不同而有所差异。
对于所测试的菌株，桔霉素的产生似乎是一个稳定的特性。随着时间的推移，硅胶保存和冷冻干燥时棒曲霉素的检测趋势明显更一致，尤其是当菌株在GJ上生长时。
所测试霉菌毒素图谱的变异性似乎更多地是菌株特异性的，而非取决于所使用的保藏技术。
