培养保存技术对扩展青霉（Penicillium expansum Link.）产棒曲霉素和桔霉素的影响

The effect of culture preservation techniques on patulin and citrinin production by Penicillium expansum Link.

作者信息

Santos I M, Abrunhosa L, Venâncio A, Lima N

机构信息

Micoteca da Universidade do Minho and Centro de Engenharia Biológica da Universidade do Minho, Braga, Portugal.

出版信息

Lett Appl Microbiol. 2002;35(4):272-5. doi: 10.1046/j.1472-765x.2002.01186.x.

DOI:10.1046/j.1472-765x.2002.01186.x

PMID:12358686

Abstract

AIMS

To study the influence of culture preservation methods and culture conditions on the production of the mycotoxins patulin and citrinin by Penicillium expansum.

METHODS AND RESULTS

Ten strains of Penicillium expansum were preserved using subculture and maintenance at 4 degrees C, mineral oil, drying on silica gel and freeze-drying. Patulin and citrinin production was assessed on yeast extract sucrose agar (YES) and grape juice agar (GJ), using TLC before and after 0.5, 2-3, 6 and 12 months preservation. Citrinin was detected in all cultures for all preservation techniques on YES. The patulin profiles obtained differed with strain and culture media used.

CONCLUSIONS

Citrinin production seems to be a stable character for the tested strains. There is a tendency for patulin detection with time apparently more consistent for silica gel storage and freeze-drying, especially when the strains are grown on GJ.

SIGNIFICANCE AND IMPACT OF THE STUDY

Variability in the profiles of the mycotoxins tested seems to be more strain-specific than dependent on the preservation technique used.

摘要

目的

研究保藏方法和培养条件对扩展青霉产生棒曲霉素和桔霉素这两种霉菌毒素的影响。

方法与结果

采用传代培养并于4℃保存、矿物油、硅胶干燥和冷冻干燥等方法对10株扩展青霉进行保藏。在酵母提取物蔗糖琼脂（YES）和葡萄汁琼脂（GJ）上评估棒曲霉素和桔霉素的产生情况，在保藏0.5、2 - 3、6和12个月前后使用薄层色谱法进行检测。在YES上，所有保藏技术的所有培养物中均检测到桔霉素。所获得的棒曲霉素图谱因菌株和所用培养基的不同而有所差异。