Wullems G J, van der Horst J, Bootsma D
Somatic Cell Genet. 1975 Apr;1(2):137-51. doi: 10.1007/BF01538544.
Evidence is presented for the uptake of radioactive-labeled isolated Chinese hamster chromosomes following incubation with Chinese hamster cells. Metaphases were found which contained radioactive labeled chromosomes in a very low frequency, and in some of the labeled chromosomes only one chromatid was labeled. Incubation of hypoxanthine phosphoribosyltransferas (HPRT)-deficient Chinese hamster cells with chromosomes isolated from HPRT+ Chinese hamster or human cells resulted in the appearance of HPRT+ cells. Clones derived from these cells were isolated in HAT medium. Cells in mitosis during incubation with the chromosomes yielded thr-e times more HPRT+ clones than did cells in interphase. The intraspecies combination involving recipient cells and chromosomes from Chinese hamster origin yielded significantly higher numbers of HPRT+ clones than did the interspecies system using human chromsomes and Chinese hamster recipient cells (5 X 10(-5) and 6 X 10(-6) respectively). Electrophoresis of HPRT from Chinese hamster cells treated with human chromosomes revealed the pattern of the human enzyme.
有证据表明，将放射性标记的分离中国仓鼠染色体与中国仓鼠细胞一起孵育后，会发生摄取现象。发现中期相中含有放射性标记染色体的频率非常低，并且在一些标记染色体中只有一条染色单体被标记。将次黄嘌呤磷酸核糖转移酶（HPRT）缺陷的中国仓鼠细胞与从HPRT +中国仓鼠或人类细胞分离的染色体一起孵育，会导致出现HPRT +细胞。从这些细胞衍生的克隆在HAT培养基中分离出来。与染色体孵育期间处于有丝分裂期的细胞产生的HPRT +克隆比间期细胞多三倍。涉及来自中国仓鼠来源的受体细胞和染色体的种内组合产生的HPRT +克隆数量明显高于使用人类染色体和中国仓鼠受体细胞的种间系统（分别为5×10（-5）和6×10（-6））。用人类染色体处理的中国仓鼠细胞的HPRT电泳显示出人类酶的模式。
