Bakay B, Graf M, Carey S, Nissinen E, Nyhan W L
Biochem Genet. 1978 Apr;16(3-4):227-37. doi: 10.1007/BF00484080.
Polyethylene glycol-1000 (PEG-1000) induced fusion of HPRT (E.C. 2.4.2.8) deficient Chinese hamster cells with alpha-galactosidase A (E.C. 2.3.1.22) deficient cells from a patient with Fabry's disease yielded hybrids which contained both human and hamster HPRT, G6PD (E.C. 1.1.1.49), and APRT (E.C. 2.4.2.7) and Chinese hamster alpha-galactosidase B. Thus PEG-1000 mediated somatic cell fusion led to reexpression of Chinese hamster HPRT. It did not restore the expression of human alpha-galactosidase. Since PEG-1000 treatment of HPRT- Chinese hamster cells in the absence of human cells yielded no HPRT+ cells, it is concluded that the element responsible for the restoration of rodent HPRT was contributed by the human cells and not by the agent employed to promote fusion.
聚乙二醇-1000（PEG - 1000）诱导次黄嘌呤磷酸核糖转移酶（E.C. 2.4.2.8）缺陷的中国仓鼠细胞与法布里病患者的α - 半乳糖苷酶A（E.C. 2.3.1.22）缺陷细胞融合，产生的杂种细胞同时含有人类和仓鼠的次黄嘌呤磷酸核糖转移酶、葡萄糖 - 6 - 磷酸脱氢酶（E.C. 1.1.1.49）、腺嘌呤磷酸核糖转移酶（E.C. 2.4.2.7）以及中国仓鼠的α - 半乳糖苷酶B。因此，PEG - 1000介导的体细胞融合导致了中国仓鼠次黄嘌呤磷酸核糖转移酶的重新表达。它并未恢复人类α - 半乳糖苷酶的表达。由于在不存在人类细胞的情况下，用PEG - 1000处理次黄嘌呤磷酸核糖转移酶缺陷的中国仓鼠细胞未产生次黄嘌呤磷酸核糖转移酶阳性细胞，所以得出结论：负责恢复啮齿动物次黄嘌呤磷酸核糖转移酶的元件是由人类细胞提供的，而非用于促进融合的试剂。
