Minna J D, Marshall T H, Shaffer-Berman P V
Somatic Cell Genet. 1975 Oct;1(4):355-69. doi: 10.1007/BF01538667.
Hybrid cells are readily formed by fusing clonal Chinese hamster cells to fresh, noncultured, adult mouse spleen cells followed by isolation in selective medium. The vast majority of such hybrids retain Chinese hamster chromosomes and isozymes while segregating mouse chromosomes and isozymes. The growth, plating efficiency, ease of karyology, and rapid segregation of mouse markers allows linkage tests in primary clones. Analysis of 13 isozymes showed 12 to be asyntenic and on epair (PGD-PGM2) to be syntenic This system will allow extensive somatic cell hybrid gene mapping in the mouse and permit a comparison of human and mouse linkage relationships.
通过将克隆的中国仓鼠细胞与新鲜的、未培养的成年小鼠脾细胞融合，然后在选择性培养基中进行分离，很容易形成杂交细胞。绝大多数这样的杂种保留了中国仓鼠的染色体和同工酶，同时分离出小鼠的染色体和同工酶。小鼠标记物的生长、接种效率、核型分析的简易性以及快速分离，使得在原代克隆中进行连锁测试成为可能。对13种同工酶的分析表明，其中12种是非同线的，一对（PGD-PGM2）是同线的。这个系统将允许在小鼠中进行广泛的体细胞杂交基因定位，并允许比较人类和小鼠的连锁关系。
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