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[中国仓鼠与美国水貂体细胞杂种的分离与特性]

[Isolation and characteristics of somatic cell hybrids of the Chinese hamster and American mink].

作者信息

Rubtsov N B, Radzhabli S I, Gradov A A, Serov O L

出版信息

Tsitol Genet. 1981;15(3):54-8.

PMID:6942558
Abstract

The paper deals with obtaining somatic cell hybrids of Chinese hamster and mink by means of inactivated Sendy virus. 39 hybrid clones segregating mink chromosomes were formed by fusing Chinese hamster cells deficient in hypoxanthine phosphoribosyliransferase with normal cells of mink. Enzyme analyses of these hybrid clones revealed that in mink genes coding lactate dehydrogenase-A, lactate dehydrogenase-B, malate dehydrogenase-NAD (soluble), 6-phosphogluconate dehydrogenase, glucose-6-phosphate dehydrogenase are not syntenic. A possibility of successful utilization of these somatic cell hybrids for mapping mink genes is shown.

摘要

本文论述了通过灭活仙台病毒获得中国仓鼠和水貂体细胞杂种的方法。将缺乏次黄嘌呤磷酸核糖基转移酶的中国仓鼠细胞与正常水貂细胞融合,形成了39个分离水貂染色体的杂种克隆。对这些杂种克隆的酶分析表明,在水貂中,编码乳酸脱氢酶-A、乳酸脱氢酶-B、苹果酸脱氢酶-NAD(可溶性)、6-磷酸葡萄糖酸脱氢酶、葡萄糖-6-磷酸脱氢酶的基因不是同线的。展示了成功利用这些体细胞杂种来绘制水貂基因图谱的可能性。

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