Koizumi T, Hendel E, Lalley P A, Tchetgen M B, Nadeau J H
Laboratory Animal Center, Fukui Medical School, Japan.
Mamm Genome. 1995 Apr;6(4):263-8. doi: 10.1007/BF00352413.
To enhance the comparative map for human Chromosome (Chr) 13, we identified clones for human genes and anonymous loci that cross-hybridized with their mouse homologs and then used linkage crosses for mapping. Of the clones for four genes and twelve anonymous loci tested, cross-hybridization was found for six, COL4A1, COL4A2, D13S26, D13S35, F10, and PCCA. Strong evidence for homology was found for COL4A1, COL4A2, D13S26, D13S35, and F10, but only circumstantial homology evidence was obtained for PCCA. To genetically map these mouse homologs (Cf10, Col4a1, Col4a2, D14H13S26, D8H13S35, and Pcca-rs), we used interspecific and intersubspecific mapping panels. D14H13S26 and Pcca-rs were located on the distal portion of mouse Chr 14 extending by approximately 30 cM the conserved linkage between human Chr 13 and mouse Chr 14, assuming that Pcca-rs is the mouse homolog of PCCA. By contrast, Cf10, Col4a1, Col4a2, and D8H13S35 mapped near the centromere of mouse Chr 8, defining a new conserved linkage. Finally, we identified either a closely linked sequence related to Col4a2, or a recombination hot-spot between Col4a1 and Col4a2 that has been conserved in humans and mice.
为了完善人类13号染色体（Chr）的比较图谱，我们鉴定了与小鼠同源基因发生交叉杂交的人类基因和无名基因座的克隆，然后利用连锁杂交进行图谱绘制。在检测的4个基因和12个无名基因座的克隆中，发现6个存在交叉杂交，即COL4A1、COL4A2、D13S26、D13S35、F10和PCCA。发现COL4A1、COL4A2、D13S26、D13S35和F10有很强的同源性证据，但仅获得PCCA的间接同源性证据。为了对这些小鼠同源基因（Cf10、Col4a1、Col4a2、D14H13S26、D8H13S35和Pcca-rs）进行遗传图谱绘制，我们使用了种间和亚种间图谱绘制面板。假设Pcca-rs是PCCA的小鼠同源基因，D14H13S26和Pcca-rs位于小鼠14号染色体的远端部分，将人类13号染色体和小鼠14号染色体之间的保守连锁延伸了约30厘摩。相比之下，Cf10、Col4a1、Col4a2和D8H13S35定位于小鼠8号染色体着丝粒附近，定义了一个新的保守连锁。最后，我们鉴定出一个与Col4a2紧密连锁的序列，或者是Col4a1和Col4a2之间一个在人类和小鼠中都保守的重组热点。
