Smith P M, Lakatos A, Barnett S C, Jeffery N D, Franklin R J M
Department of Clinical Veterinary Medicine, University of Cambridge, Madingley Road, United Kingdom.
Exp Neurol. 2002 Aug;176(2):402-6. doi: 10.1006/exnr.2002.7936.
Naturally occurring spinal cord injury in dogs provides a potentially powerful intermediate model for testing the efficacy of therapeutic strategies developed in experimental rodent models before phase 1 trials in human patients. A particularly promising strategy involves transplantation of olfactory ensheathing cells (OECs) that both promote axon regeneration and generate new myelin sheaths. As a first step in developing OEC transplantation in the canine intermediate model we describe the isolation, purification, and characterization of OECs from adult dog olfactory bulb. We also show that the canine OEC behaves in a manner similar to its rodent counterpart following transplantation into demyelinating lesions in rat spinal cord and that these properties are retained following cryopreservation.
犬类自然发生的脊髓损伤为在人类患者进行1期试验之前，测试在实验性啮齿动物模型中开发的治疗策略的疗效提供了一个潜在的强大中间模型。一种特别有前景的策略涉及移植嗅鞘细胞（OECs），这种细胞既能促进轴突再生，又能生成新的髓鞘。作为在犬类中间模型中开发OEC移植的第一步，我们描述了从成年犬嗅球中分离、纯化和鉴定OECs的过程。我们还表明，将犬类OEC移植到大鼠脊髓的脱髓鞘损伤部位后，其行为方式与其啮齿动物对应物相似，并且这些特性在冷冻保存后仍然保留。
