Department of Anatomy and Embryology, Peking University Health Science Center, Beijing, China 100191.
Brain Res. 2010 Mar 4;1317:257-67. doi: 10.1016/j.brainres.2009.12.061. Epub 2010 Jan 4.
Trials of neuroprotection in ischemic stroke mainly concern gray matter protection, with little information on white matter damage. Olfactory ensheathing cells (OECs) are capable of providing continuous neurotrophic factor and of promoting growth and remyelination of damaged axons. We evaluated OECs for the repair of white matter after transplantation in middle cerebral artery occlusion (MCAO) rat. OECs were cultured from the olfactory bulbs of adult green fluorescent protein (GFP)-expressing transgenic rats and transplanted into peri-infarct basal ganglia imminently after reperfusion. The mortality, neurological score, and function were record everyday until 56 days. At 24 h and 56 days, the infarction volume, the Luxol-fast blue (LFB) staining, and Neurofilament (NF) immunohistochemistry and Western blot were performed to assess the demyelination and remyelination in white matter. OECs transplantation reduced the infarct volume, decreased mortality, and improved neurological deficits in 56 days after MCAO. LFB marked myelin, NF-positive immunohistochemistry, and Western blot indicated that the remyelination and axon regeneration in OEC transplanted rat were significant. In conclusion, OECs can protect the white matter from ischemic injury, but the potential mechanisms of transplanted OEC-mediated recovery need further studies to identify.
在缺血性脑卒中的神经保护试验中，主要关注灰质的保护，而对白质损伤的信息了解较少。嗅鞘细胞（OECs）能够提供持续的神经营养因子，并促进受损轴突的生长和髓鞘再生。我们评估了嗅鞘细胞在大脑中动脉闭塞（MCAO）大鼠移植后对白质修复的作用。从成年 GFP 表达转基因大鼠的嗅球中培养 OECs，并在再灌注后即刻移植到梗死周边基底节区。每天记录死亡率、神经评分和功能，直到 56 天。在 24 小时和 56 天时，进行脑梗死体积、卢索快蓝（LFB）染色、神经丝（NF）免疫组化和 Western blot 检测，以评估白质脱髓鞘和髓鞘再生情况。OEC 移植可减少 MCAO 后 56 天的梗死体积、降低死亡率和改善神经功能缺损。LFB 标记的髓鞘、NF 阳性免疫组化和 Western blot 表明 OEC 移植大鼠的髓鞘再生和轴突再生明显。总之，OECs 可以保护白质免受缺血性损伤，但需要进一步研究来确定移植的 OEC 介导恢复的潜在机制。
