Péterfy F, Miholics E, Boros B
Ann Immunol Hung. 1975;18:123-9.
The authors describe a simple method for isolating heterogeneous IgM from fraction I obtained as a by-product of the large scale fractionation of human plasma. The optimum circumstances of fractionation were determined and it was found that the majority of IgM is precipitated between 2 and 5% polyethylene glycol concentration and 1.1 and 1.8 M ammonium sulphate concentration. After a simple fractionation and gel filtration, a preparation of high purity can be obtained.
作者描述了一种从人血浆大规模分级分离的副产品Ⅰ组分中分离异质性IgM的简单方法。确定了分级分离的最佳条件，发现大多数IgM在聚乙二醇浓度为2%至5%、硫酸铵浓度为1.1至1.8M时沉淀。经过简单的分级分离和凝胶过滤后，可获得高纯度的制剂。
