使用聚乙二醇沉淀法和亲和色谱法从正常血清中分离人IgA和IgM。
Isolation of human IgA and IgM from normal serum using polyethylene glycol precipitation and affinity chromatography.
作者信息
Cripps A W, Neoh S H, Smart I J
出版信息
J Immunol Methods. 1983 Feb 25;57(1-3):197-204. doi: 10.1016/0022-1759(83)90078-9.
A simple method is described for the preparation of highly purified IgA and IgM from small volumes of human serum. Enriched IgM and IgA fractions were prepared by precipitation with 7% (w/v) and 14% (w/v) polyethylene glycol respectively. This was followed by affinity chromatography and gel filtration. The final recovery of both IgA and IgM was approximately 30%. The purified preparations obtained were characterized by immunoelectrophoresis, double immunodiffusion, radial immunodiffusion, radioimmunoassay and gel filtration.
描述了一种从少量人血清中制备高度纯化的IgA和IgM的简单方法。分别通过用7%（w/v）和14%（w/v）聚乙二醇沉淀来制备富集的IgM和IgA组分。随后进行亲和层析和凝胶过滤。IgA和IgM的最终回收率约为30%。通过免疫电泳、双向免疫扩散、放射免疫扩散、放射免疫测定和凝胶过滤对获得的纯化制剂进行了表征。
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