Fekete M, Kovács M, Tollas G
Ann Immunol Hung. 1975;18:229-36.
It appears from the experiments of the authors that the macroscopic changes taken place during freezing and heating could be explained by the formation of an eutectic crystalline structure in the case of human plasma and albumin solutions. The temperature interval of eutectic spot formation showed good agreement with the temperature range of initial thawing as determined by the DTA method, i.e. with the working zone freeze-drying. It was possible to determine the maximum temperature of complete solidification from the resistance curves of the haemoderivatives. Judging from the shape of the resistance curves, the phenomenon of undercooling did not emerge with the freezing rate applied. Examining the optimal freezing rate, it appeared on the basis of some quality characteristics of the freeze-dried end-product, that the best results were obtained with --30 degrees C freezing in the case of albumin and plasma, and with --45 degrees C freezing in the case of normal and iv immunoglobulins. But the authors deem it necessary to conduct further experiments in this respect.
从作者的实验来看，在人血浆和白蛋白溶液中，冷冻和加热过程中发生的宏观变化可以用共晶晶体结构的形成来解释。共晶点形成的温度区间与差示热分析法测定的初始解冻温度范围吻合良好，即与冷冻干燥的工作区吻合。根据血液衍生物的电阻曲线可以确定完全凝固的最高温度。从电阻曲线的形状判断，在所采用的冷冻速率下未出现过冷现象。在研究最佳冷冻速率时，基于冻干终产品的一些质量特性，发现白蛋白和血浆在-30℃冷冻时效果最佳，正常免疫球蛋白和静脉注射免疫球蛋白在-45℃冷冻时效果最佳。但作者认为有必要在这方面进行进一步实验。
